Description
© Liofilchem® - H2O2 / Catalase Reagent - Rev.1 / 13.05.2024
Instructions For Use
Description
H O / Catalase Reagent is a reagent for microbiological use made of a H O solution, for the performance of the 2 2 2 2 catalase test. KIT CONTENT • 1 vial of H O solution (5 ml) 2 2 METHOD PRINCIPLE Hydrogen peroxide, final product of aerobic metabolism of carbohydrates, is extremely toxic for bacteria. Catalase-positive microorganisms, containing the enzyme catalase, break down hydrogen peroxide into water and oxygen. Except for Streptococci, most of aerobe and facultative anaerobe microorganisms have catalase activity. Catalase reagent shows the presence of enzyme catalase with gas bubbles production. MATERIALS REQUIRED BUT NOT PROVIDED Standard microbiological supplies and equipment such as: inoculating loop, pipettes, test tube, culture media, Catalase / Oxy Test, physiological solution (0.85% saline), quality control organisms. REAGENTS • A vial of H O / Catalase Reagent contain 0.375 ml di H O dissolved in 4.625 mL distilled water. 2 2 2 2 SPECIMEN Collect specimens in sterile containers or with sterile swabs and transport to the laboratory. Process each specimen using procedures appropriate for that sample. This product is recommended for use only with pure cultures. Refer to specific guidelines for more detailed information. TEST PROCEDURE 1. Take the number of H O / Catalase Reagent needed from the fridge and allow them to reach room temperature. 2 2 2. Add 4 drops of physiological solution (0.85 % saline) to each tube. 3. With a sterile loop pick up one or more than one well isolated colony from a fresh culture plate (18-24 hours old) and emulsify in the tube. 4. Add 3 drops of H O Reagent. 2 2 5. Observe the almost immediate development of gas bubbles and the foam formation in the tube. INTERPRETING RESULTS The production of catalase enzyme is evidenced by the immediate bubbles and foam formation after the addition of H O / Catalase Reagent to a suspension of the microorganism. 2 2 The absence of bubbles and foam formation indicates a microorganism unable to resolve H O , therefore lacking in 2 2 catalase enzyme.
Specifications
- Storage: the expiry date indicated on the label
- Shelf life: 3 years
Payment & Security