Description
Skim Milk
Powder skim milk for preparing microbiological culture media.
Description
Skim Milk can be used to prepare Skim Milk Agar for detecting proteolytic microorganisms in foods, including dairy products. It can also be used to prepare Litmus Milk, a differential test medium for determining lactose-fermentation and for detecting proteolytic enzymes that hydrolyze casein and cause coagulation. This medium is particularly suitable for differentiating Clostridium species. When prepared in a 10% solution, it is equivalent to fresh skim milk and is used for the cultivation of lactobacillus.
Principle
Skim Milk is a source of lactose and casein in prepared culture media.
Preparation
- Mix the powder to a smooth paste with a small quantity of distilled water, then gradually add more distilled water until a 10% w/v mixture is obtained
- Sterilize at 121°C for 5 minutes
- The solution can be added to a culture medium or used by itself.
Technique
Refer to appropriate references for specific procedures using Skim Millk.
Interpretation of Results
Refer to appropriate references for test results.
Specifications
- Form: Dehydrated medium
- Storage: 10-30°C
- Shelf life: 4 years
Payment & Security