Description
Powdered skim milk for preparing microbiological culture media.
Description
Skim Milk can be used to prepare Skim Milk Agar for detecting proteolytic microorganisms in foods, including dairy products. It can also be used to prepare Litmus Milk, a differential test medium for determining lactose fermentation and for detecting proteolytic enzymes that hydrolyze casein and cause coagulation — particularly suitable for differentiating Clostridium species. When prepared as a 10% solution, it is equivalent to fresh skim milk and is used for cultivating lactobacilli.
Principle
Skim Milk is a source of lactose and casein in prepared culture media.
Preparation
- Mix the powder to a smooth paste with a small quantity of distilled water, then gradually add more distilled water until a 10% w/v mixture is obtained
- Sterilize at 121°C for 5 minutes
- The solution can be added to a culture medium or used by itself
Technique & Interpretation of Results
Refer to the applicable reference method for your laboratory's specific procedures and result interpretation.
Storage & Shelf Life
Dehydrated medium: store at 10–30°C. Shelf life: 4 years.
Manufacturer catalogue code: 610491.
Payment & Security