Description
© Liofilchem® - Tryptic Soy Agar - EN ISO 11133 - Rev.1 / 28.02.2020
Instructions For Use
Description
Tryptic Soy Agar (TSA) is a non selective isolation medium used for the growth of bacteria which do not have specific nutritional requirements and for the preparation of reference strains with the aim of growth promotion tests of culture media. This medium complies with EN ISO 11133 for microbiological examination of food, animal feed and water, where it is described as the main reference medium to carry out quantitative and qualitative testing of specific culture media. Tryptic Soy Agar is also recommended in the harmonized chapters of the United States (USP), European (EP) and Japanese Pharmacopoeia (JP). For the usage in Pharmaceutical Industry, Liofilchem offers products having the same composition as TSA described in the ISO standard, but which are specifically controlled according to the Pharmacopoeial performance requirements. See the IFU available for the product ref. number 10037S.
Preparation
- Dehydrated medium Suspend 40 g of the powder in 1 liter of distilled or deionized water
- Heat to boil shaking frequently until completely dissolved
- Sterilize in autoclave at 121°C for 15 minutes
- If desired, add appropriate volume of sterile defibrinated blood for preparing 5 to 10% blood agar
- Medium in tubes/ Melt the content of the tube/bottle in a water bath at 100°C (loosing the cap partially bottles removed) until completely dissolved
- Then screw the cap and check the homogeneity of the dissolved medium, if it is the case turning the tube/bottle upside down
- Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into Petri dishes
- TEST PROCEDURE Perform serial dilutions of the test sample in order to achieve a colony count of between 15 and 300 colonies per plate
- Use a suitable diluent such as Buffered Peptone Water (ref
- 24099) or Maximum Recovery Broth (ref
- Inoculate the medium by pour plating, spread/streak method or membrane filtration.
Specifications
- Form: Tryptic Soy Agar
- Storage: 2
- Shelf life: Dehydrated medium: 4 years
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