Product and use case
Liofilchem BRILLIANT Green Bile Broth 2% Culture Media 620010 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is brilliant Green Bile Broth 2% is a liquid medium used for the detection or confirmation of coliform bacteria in water and wastewater, foods, dairy products and other materials of sanitary importance. Brilliant Green Bile Broth 2% is formulated according to APHA, ISO 4831 and ISO 4832.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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When this format is useful
Use this product where indicator organisms are part of a release, trend, or sanitation decision. The practical value comes from separating countable or presumptive target reactions from background flora so the analyst can document whether the sample meets the site limit.
What to read after incubation
Turbidity and gas production indicate coli-aerogenes organisms.
Selective or enrichment logic
Enzymatic digest of casein provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Lactose is the fermentable carbohydrate. Ox bile and brilliant green inhibit Gram-positive bacteria and many Gram-negative bacteria, other than coliforms.
Sample handling workflow
- Preparation: Dehydrated medium Suspend 40.0* g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. Distribute into 10 ml tubes with Durham gas collecting tube. Sterilize in autoclave at 121°C for 15 minutes. *Dissolve 80.0 g of the power in 1 liter of distilled or deionized water to make the double strength broth. Medium in bottles Aseptically, dispense the medium into tubes fitted with Durham tubes.
- Procedure: 1.For coliforms detection, inoculate tubes with 1 ml diluted or undiluted sample (use double strength broth for larger volume samples). For use in the confirmation of presumptive tests, subculture from Lauryl Sulphate Tryptose Broth (ref. 21453) or from typical coliform colonies on Violet Red Bile Lactose Agar (ref. 11183). 2.To indicate the presence of Escherichia coli, incubate at 44 ± 1°C for 48 hours. ISO 4831 and ISO 4832 recommend to incubate at 30°C or 37°C for 24-48 hours. 3.Examine tubes for gas formation.
Technical verification points
- Formula / composition: (g/l) Enzymatic Digest of Casein 10.0 Lactose 10.0 Ox Bile 20.0 Brilliant Green 0.0133 Final pH 7.2 ± 0.2 at 25°C
- Quality control: The tubes are inoculated with the microbial strains indicated in the QC table. Inoculum for productivity: ≤100 CFU. Inoculum for selectivity: >103 CFU. Incubation conditions: 30 ± 1°C for 24-48 hours. QC Table. Microorganism Growth Gas Escherichia coli WDCM 00012 Good + Escherichia coli WDCM 00013 Good + Citrobacter freundii WDCM 00006 Good + Enterococcus faecalis WDCM 00009 Partially to completely inhibited − Enterococcus faecalis WDCM 00087 Partially to completely inhibited − WARNING AND
Storage, hazards, and disposal
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for professional use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store bottles and tubes at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be sp ecially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74620010
- Catalog/document code matched: 620010, 2018, 4831, 4832
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/brilliant-green-bile-broth-2-for-microbiology-lab-testing