Laboratory use profile
Liofilchem LB Medium (Luria Bertani) Culture Media 610084 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is l.B.MEDIUM (LURIA BERTANI) is a dehydrated medium used for maintaining and cultivating of recombinat strains of Escherichia coli in molecular biology procedures.These strains are generally derived from E.coli K12, which are deficient in B vitamin production. This strain of E.coli has been further modification throught specific mutation to create an auxotrophic strain that is not capable of growth on nutrionally deficient media.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Use case and sample types
Use this product where indicator organisms are part of a release, trend, or sanitation decision. The practical value comes from separating countable or presumptive target reactions from background flora so the analyst can document whether the sample meets the site limit.
Decision criteria after incubation
Growth is evident by the appearance of turbidity.
Method logic
L.B.MEDIUM (LURIA BERTANI) provides all the nutritional requirements of recombinat strains of Escherichia coli. Peptone provides nitrogen and carbon. Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride.
Preparation and execution notes
- Preparation: Suspend 20.0 g of powder in 1 litre of distilled or deionized water. Mix until completely dissolved. Dispense into final containers. Sterilize in autoclave at 121°C for 15 minutes.
- Procedure: Consult appropriate
Formula, QC, and performance
- Formula / composition: (g/L) Tryptone............................................................................... 10.0 Yeast extract ……………..……………………………........... 5.0 Sodium chloride................................................................... 5.0 Final pH 7.0 ± 0.2
Safety and documentation points
- Hazards: 2.1 Classificatio n of the substance or mixture This product does not meet the criteria for classif ication in any hazard class in accordance to Regula tion (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label eleme nts The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610084
- Catalog/document code matched: 610084
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/lb-medium-luria-bertani-for-microbiology-lab-testing