Application snapshot
Liofilchem Tryptic (CTA) Medium Culture Media 610185 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is tRYPTIC (CTA) MEDIUM is a semisolid medium used with added carbohydrates in differentiating microorganisms based on fermentation reactions and motility. Fermentation of the test carbohydrate is observed when acid is formed and the medium turns from red to yellow. Motility of an organism is evident as a haze of growth extending into the agar from the stab line.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Application fit for the lab
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Expected colonies or reaction pattern
free-flowing, homogeneous. Color: pink. Prepared medium
How to build it into the SOP
- Procedure: Heavily inoculate the specimen by stabbing using a sterile needle and incubate at 36 ± 1°C for 18-24 hours.
Composition and QC checkpoints
- Formula / composition: (g/l) Tryptose 20.0 L-Cystine 0.5 Sodium Chloride 5.0 Sodium Sulphite 0.5 Phenol Red 0.017 Agar 2.5 Final pH = 7.3 ± 0.2 at 25 °C. DIRECTIONS Suspend 28.5 g of powder in 1 liter of distilled or deionized water. Heat to boiling until completely dissolved. Dispense into 10 ml tubes and sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C. Aseptically add a sterile solution of carbohydrate at 0.5-1% final concentration. Mix well.
Handling limits and documentation
- Limitations: Neisseria species usually produce acid only in the area of stabs. If there is a strong acid (yellow colour) throughout the medium, a contaminating organism may be present. If in doubt about a tube containing a Neisseria species, a Gram stain and oxidase test should be performed on the growth.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8 °C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods SAFETY DAT A SHEET (According to Regulation EC n ° 1907/2006) Revision: 3 / Revision Date: 01.06.201 5 Liofilchem s.r.l. Via Scozia - Zona Industriale 64026, Roseto degli Abruzzi (TE) Italy - Tel +39 0858930745 - Fax +39 0858930330 MSDS Ref. 610185-620185 Page 4 of 5 Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative.
Before adding it to the cart
- Kormay SKU: KB74610185
- Catalog/document code matched: 610185
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/tryptic-cta-medium-for-microbiology-lab-testing