Product overview
Liofilchem BRYANT AND BURKEY Medium Culture Media 610142 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is bRYANT AND BURKEY MEDIUM is a medium or the enumeration of lactate- fermenting spore-forming anaerobes in milk and dairy products.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Where this product fits
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Result signals and confirmation
Tubes showing both growth and gas production (indicated by upward movement of the paraffin more than 1 cm) are considered positive for the presence of lactate -fermenting clostridial spores. Determine the spore count using the Most Probable Number (MPN) method.
Method principle
Tryptone, meat extract and L-cysteine provide nutrients and cofactors required for good growth of clostridia. Yeast extract supplies B-complex vitamins which stimulate bacterial growth. Selectivity of this medium is achieved through the addition of sodium acetate which is also the principal promoter of spores germination.
Bench workflow
- Preparation: Suspend 38.0 g of powder in 1 litre of distilled or deionized water. Heat until completely dissolved. Dispense 10 mL amounts into tubes. Sterilize in the autoclave at 121 °C for 15 minutes.
- Procedure: 1. Prepare tenfold dilutions of the sample and inoculate triplicate tubes of BRYANT AND BURKEY MEDIUM with 1 mL of each sample dilution. 2. Pour approximatively 2 mL of melted paraffin (60-65°C), previously autoclaved at 121°C for 20 minutes, into each tube. 3. Heat the tubes to 75°C to kill vegetative cells and activate spores; allow to cool to room temperature. 4. Incubate tubes at 35°C for 6 days. 5. Examine tubes for growth and gas production after 48 hours incubation and daily for up to 6 days.
Controls and performance checks
- Formula / composition: (g/L) Tryptone 15.0 Yeast Extract 5.0 Meat Extract 7.5 Sodium Acetate 5.0 L- Cysteine 0.5 Resazurin 0.002 Calcium Lactate 5.0 Final pH =5.9 ± 0.2 at 25°C
Storage, limits, and safety
- Warnings / precautions: The product is not classified as hazardous by current legislation and does not contain harmful substances in concentrations of ≥1%. It is nevertheless recommended that the Safety Data Sheet be consulted on its correct use. The product must be used only by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared media at 2-8°C. WARNING and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610142
- Catalog/document code matched: 610142
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/bryant-and-burkey-medium-for-microbiology-lab-testing