Product and use case
Liofilchem TSI Agar acc. to E.P. Culture Media 610339 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is t.S.I. AGAR acc. to E.P. is a medium recommended for the detection and enumeration of enterobacteria, formulated according to European Pharmacopoeia.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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When this format is useful
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
What to read after incubation
Fermentation of the sugars is shown with change to yellow of phenol red indicator. The glucose concentration is 1/10 of that lactose and saccharose, for an earlier detection of bacteria that ferment only glucose. Fermentation of glucose determines on surface (where are aerobic conditions) a production of ammonium ions and a red colour (alkaline pH) of phenol red indicator; while in the butt, where are anaerobic conditions, the glucose fermentation determines the production of acids and change to yellow (acid pH) of phenol red indicator.
Selective or enrichment logic
Meat Extract and Peptone provide nitrogen and other nutrient to support microbial growth. Yeast Extract provides amino acids and vitamins of group B. Sodium Chloride maintains the osmotic balance of the medium. Lactose, glucose and saccharose are a source of energy which allows the differentiation of microorganisms. Neutral Red is a pH indicator. Sodium thiosulphate and ammonium ferric citrate are the substrate for the production of H2S. Agar is the solidifying agent.
Sample handling workflow
- Preparation: Suspend 68.0 g of powder in 1 litre of distilled or deionized water. Heat until completely dissolved. Sterilize in the autoclave at 121 °C for 15 minutes. Cool down to 45-50 °C. Dispense in final tubes and allow to solidify in a slant position.
- Procedure: Touch a well-isolated colony to test using an inoculum needle. Inoculate the medium by stabbing the butt and streaking the slope. Incubate the tubes at 36 ± 1 °C for 24-48 hours, with the caps loosened to favour gas exchange.
Technical verification points
- Formula / composition: (g/L) Meat Extract 3.0 Peptone 20.0 Yeast Extract 3.0 Sodium Chloride 5.0 Lactose 10.0 Glucose 1.0 Saccharose 10.0 Neutral Red 0.03 Sodium Thiosulphate 0.3 Ammonium Ferric Citrate 0.3 Agar 15.0 Final pH = 7.4 ± 0.2 at 25°C.
Storage, hazards, and disposal
- Warnings / precautions: The product is not classified as hazardous by current legislation and does not contain harmful substances in concentrations of ≥1%. It is nevertheless recommended that the Safety Data Sheet be consulted on its correct use. The product must be used only by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared media at 2-8°C. WARNING and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classif ication in any hazard class in accordance to Regula tion (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610339
- Catalog/document code matched: 610339
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/tsi-agar-acc-to-e-p-for-microbiology-lab-testing