Laboratory use profile
Liofilchem CHAPMAN STONE Medium Culture Media 610353 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is cHAPMAN STONE MEDIUM is a selective and differential medium for the isolation of staphylococci in foods.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Use case and sample types
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Decision criteria after incubation
Examine for growth and the presence or absence of clear zone around colonies. To determine mannitol fermentation add a few drops ofBromcresol purple to areas of the medium from which colonies have been removed. The staphylococcal colonies ferment mannitol andare coagulase- and gelatinase-positive, therefore when colony site change color to yellow after application of Bromcresol purple(positive mannitol fermentation) and the colony is surrounded by a clear zone (positive gelatinase activity) it is probably phatogenicStaphylococcus.
Method logic
Tryptone and yeast extract provide nitrogen, vitamins, minerals and amino acids essential for growth. D-Mannitol is the fermentablecarbohydrate providing carbon and energy. Gelatin and ammonium sulfate serve to determine gelatinase activity. Sodium chloride act asselective agent because most bacteria species, except staphylococci, are inhibited by high salt content. Bacteriological agar is thesolidifying agent.
Preparation and execution notes
- Preparation: Suspend 202.5 g of powder in 1000 ml of distilled or deionized water. Heat and gently agitate until completely dissolved. Sterilize inautoclave at 121°C for 15 minutes. Cool to 45-50°C, mix well and dispense in petri dishes.
- Procedure: Streak a sample of the specimen onto the surface of the agar. Make several stabs into the medium along the streak. Incubateaerobically at 30 ± 2°C for up to 48 hours.
Formula, QC, and performance
- Formula / composition: (g/l)Tryptone..............................................10.0Yeast Extract.......................................2.5Gelatin.................................................30.0D-Mannitol...........................................10.0Sodium Chloride..................................55.0Ammonium Sulfate..............................75.0Dipotassium Phosphate......................5.0Agar.....................................................15.0Final pH 7.0 ± 0.2
- Quality control: 1.Control of general characteristics, label and print2.Sterility control7 days at 25 ± 1°C, in aerobiosis7 days at 36 ± 1°C, in aerobiosis3.Microbiological controlInoculum for productivity: 10-100 UFC/mlInoculum for selectivity: 104-105 UFC/mlInoculum for specificity: ≤104 UFC/mlIncubation Conditions: 18-48 h at 30 ± 2°C, in aerobiosisMicroorganismsGrowthHalo (Gelatinase)Mannitol fermentationEscherichia coli ATCC 25922Inhibited--Streptococcus aureusATCC 25923Good++Staphylococcus epidermidisATCC 12228Good+-TABLE OF SYMBOLS Batch code Keep away fromsunlight Manufacturer Use by Cataloguenumber
Risk controls for routine use
- Warnings / precautions: The product is not classified as hazardous by current legislation and does not contain harmful substances in concentrations of ≥1%.
- Storage / shelf life: 10-30°C away from light, until the expiry date on the label or until signs of deterioration or contamination are evident.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.REFERENCES1.Chapman. 1948. Food Res. 13:100. 2.Chapman. 1946. J. Bacteriol. 51:409. 3.MacFaddin. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria. Williams & Wilkins, Baltimore, Md.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Skin Irrit. 2, Skin irritation (Category 2); H315 Eye Irrit. 2, Eye irritation (Category 2); H319 STOT SE 3, Specific target organ toxicity - Single exposure (Category 3); H335 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H315 H319 H335 Causes skin irritation. Causes serious eye irritation.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610353
- Catalog/document code matched: 610353, 1000
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/chapman-stone-medium-for-microbiology-lab-testing