Application snapshot
Liofilchem Listeria Aesculin Agar Culture Media 11230 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is lISTERIA AESCULIN AGAR is a selective medium used for the isolation and differentiation of Listeria spp in food and environmentalsamples, according to ISO 11290.
This product supports Listeria workflows in ready-to-eat food, dairy, meat, and environmental monitoring programs where early presumptive recovery must lead into confirmation.
View this product on Kormay Biomedicals
Application fit for the lab
Use this product where the lab must protect a recovery step before confirmation. For ready-to-eat food and environmental samples, the important decision is not only whether growth appears, but whether the enrichment or plating step gives the organism enough opportunity to recover from stress, competing flora, preservatives, or cold-chain exposure.
Expected colonies or reaction pattern
Listeria spp appear concave, dark/black colored and surrounded by a halo of the same color.Suspect colonies must be submitted to Gram staining, biochemical tests, hemolysis tests on Columbia + 5% Sheep Blood, Camp test Sand Camp test R.
Principle behind the medium
Peptospecial, peptone, tryptose and maize starch supply nitrogen, carbon, amino acids and vitamins. Sodium chloride maintains theosmotic balance of the medium. Agar is the solidifying agent. The selectivity of the medium is due to cycloheximide, which inhibits thegrowth of fungi, and acriflavine, colistin, fosfomycin and cefotetan that inhibit bacterial flora. Esculin and ferric ammonium citrate allowthe presumptive identification of Listeria spp. that hydrolyze esculin to esculetin giving a dark color to the medium.
How to build it into the SOP
- Procedure: Streak a loopful of a suitable enrichment broth, incubated with the sample to be analyzed, on the surface of a plate. Invert the plate andincubate at 36+/-1° C for 24-48 hours.
Composition and QC checkpoints
- Formula / composition: (g/l)Peptospecial10.0Peptone 3.0Tryptose10.0Maize Starch 1.0Sodium Chloride 5.0Esculin 1.0Ferric Ammonium Citrate 0.5Lithium Chloride15.0Acriflavine 0.005Colistin Sulfate 0.02Cycloeximide (Actidione) 0.4Fosfomycin 0.01Cefotetan 0.002Agar15.0Final pH 7.0 ± 0.2
- Quality control: 1.Control of general characteristics, label and print2.Sterility control7 days at 22 ± 1°C, in aerobiosis7 days at 36 ± 1°C, in aerobiosis3.Microbiological controlInoculum for productivity: 10-100 CFU/mlInoculum for selectivity: 104-105 CFU/mlInoculum for specificity: !104 CFU/mlIncubation Conditions: 24-48 hours at 36±1°CMicroorganismGrowthFeaturesListeria monocytogenesATCC 19111GoodGray colonies with gray haloListeria monocytogenesATCC 35152GoodGray colonies with gray haloEscherichia coliATCC 25922Inhibited---Enterococcus faecalisATCC 29212Inhibited---Candida albicansATCC
Handling limits and documentation
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed forprofessional use only use and must be used by properly trained operators.
- Storage / shelf life: 10-25°C away from light, until the expiry date on the label. Eliminate if signs of deterioration or contamination are evident.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.REFERENCES1.TIL - IDF (1988). Provisional of Recommended Method. Milk and Milk Products. Detection Listeria Monocytogenes.2.Bortolussi R.,Schleck W.F. and Albritton W.L. (1986). “Listeria monocytogenes “ p.205-208. In Lenette E.H.,Balows A., Hausler W.J.and Shadomy H.J.(ed.) Manual of Clinical Microbiology. 4th ed.American Society for Microbiology, Washington D.C.3.ISO 11290-1:1996.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Co mponents with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB7411230
- Catalog/document code matched: 11230, 11290
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/listeria-aesculin-agar-for-microbiology-lab-testing