Laboratory use profile
Liofilchem CHINA-BLUE Lactose Agar Culture Media 610362 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is cHINA-BLUE LACTOSE AGAR is a non-selective medium used for the enumeration and differentiation of bacteria in dairy products onthe basis of their capability to ferment lactose.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Use case and sample types
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Decision criteria after incubation
The medium does not contain any inhibitory substances therefore permits luxuriant growth of all the organisms present in dairyproducts. Lactose-fermenters such as E.coli, coliform bacteria, Staphylococci and Streptococci cultivate with blue colonies. Non-lactosefermenters such as Salmonella, Serratia, Proteus species and others cultivate with colorless colonies.
Method logic
Meat extract and tryptone provide nitrogen, carbon, vitamins and minerals required for organisms growth. Sodium chloride maintains theosmotic balance of the medium. Lactose is the fermentable carbohydrate. China blue is the pH indicator that changes from colorless toblue due to degradation of lactose to acid. Agar is the solidifying agent.
Preparation and execution notes
- Preparation: Suspend 38.4 g of powder in 1 liter of distilled water. Heat until completely dissolved. Autoclave at 121°C for 15 minutes. Cool to 45°Cbefore distribute in Petri dishes.
- Procedure: Inoculate the culture medium by the streaking or pour-plate methods. Incubate at 36±1°C for 18-48 h in aerobiosis.
Formula, QC, and performance
- Formula / composition: (g/l)Meat Extract 3.0Tryptone 5.0Sodium Chloride 5.0Lactose10.0China Blue 0.375Agar15.0Final pH 7.2 ± 0.2 at 25°C
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: 10-100 CFU/mlIncubation conditions: 18-48 h at 36 ± 1°C, in aerobiosisMicroorganismGrowthColour change to blueEscherichia coliATCC 25922Good+Proteus mirabilisATCC 29906Good-Pseudomonas aeruginosaATCC 27853Good-Enterococcus faecalisATCC 19433Good+Streptococcus agalactiaeATCC 13813Good+Staphylococcus epidermidisATCC 12228Good+Bacillus cereusATCC 11778Good-TABLE OF SYMBOLS Batchcode Keep away fromheat sources Manufacturer Use by Fragile, handlewith care Cataloguenumber Temperaturelimitation
Risk controls for routine use
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product must be usedonly by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use itbefore the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8°C away fromlight.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to national and local regulations in force.REFERENCES1.Brandi E. and Sobeck-Skal E. (1963) Milchwiss. Ber. 13:1-9.2.Methodenbuch Band VI. Verband Deutscher Landwirtschaftlicher Untersuchungs und Forschungsanstalten (1970). LIOFILCHEM S.r.l.Via Scozia, Zona Ind.le - 64026, Roseto degli Abruzzi (TE) - ITALYTel +39 0858930745 Fax +39 0858930330 Website: www.liofilchem.net E-mail: liofilchem@liofilchem.net TECHNICAL SHEETTS 610362Rev.0 / 27.11.2013Page 2 of 2PRODUCT SPECIFICATIONSNAMECHINA-BLUE LACTOSE AGARPRESENTATIONDehydrated medium
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610362
- Catalog/document code matched: 610362
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/china-blue-lactose-agar-for-microbiology-lab-testing