Product and use case
Liofilchem MacConkey Agar w/o Salt Culture Media 610223 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is macConkey Agar without Salt (NaCl) is a differential medium particularly recommended for urine examination. This medium, in contrast to regular MacConkey Agar (ref. 10029), does not contain sodium chloride nor crystal violet, resulting in the prevention of Proteus species from spreading while certain Gram-positive bacteria, such as Staphylococcus and Enterococcus spp., are allowed to grow.
This product is most relevant to urine culture workflows, where colony differentiation, count interpretation, and suppression of swarming organisms help the lab move from a mixed specimen to a useful diagnostic result.
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When this format is useful
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
What to read after incubation
Lactose-nonfermenting organisms, such as Salmonella, Shigella and Proteus spp, form colorless or clear colonies. Lactose-fermenting organisms, such as E. coli and Klebsiella spp, grow as pink to red colonies with or without a zone of precipitated bile. Staphylococci cultivate with light pink-red colonies and enterococci with red colonies. NOTE: The colonial characteristics described give presumptive identification only of the isolated organisms. It is necessary to subculture and carry out confirmation tests for final identification.
Selective or enrichment logic
Peptone provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Lactose is the fermentable carbohydrate. Bile salts act as mild inhibitors of non-intestinal bacteria and contribute to inhibit the swarming of Proteus spp. Agar is the solidifying agent. Neutral red is the pH indicator.
Sample handling workflow
- Preparation: Dehydrated mediumSuspend 47.1 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes.
- Procedure: Inoculate the plates by directly streaking the specimen on the agar surface. Incubate aerobically at 35 ± 2°C for 18-24 h
Technical verification points
- Formula / composition: * (g/l) Peptone 20.0 Lactose 10.0 Bile Salts 5.0 Agar 12.0 Neutral Red 0.075 Final pH 7.4 ± 0.2 at 25°C *Adjusted and/or supplemented as required to meet
- Quality control: Plates are inoculated with the microbial strains indicated in the QC table. Inoculum for productivity: 50-100 CFU. Incubation conditions: aerobically at 35 ± 2°C for 18-24 hours. QC Table. Microorganism Growth Specification Salmonella Typhimurium ATCC 14028 Good Colorless colonies Shigella flexneri ATCC 12022 Good Colorless colonies Proteus mirabilis ATCC 12453 Good Colorless colonies Escherichia coli ATCC 25922 Good Pink colonies with bile ppt Enterococcus faecalis ATCC 29212 Good Small reddish colonies Staphylococcus aureus ATCC 25923 Good Small to medium rose colonies WARNING AND
Storage, hazards, and disposal
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for in vitro diagnostic use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store prepared plates at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610223
- Catalog/document code matched: 610223, 2019, 10029
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/macconkey-agar-w-o-salt-for-microbiology-lab-testing