Product and use case
Liofilchem BISMUTH SULPHITE Agar Culture Media 610301 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is bismuth Sulphite Agar is a medium used for the selective isolation of Salmonella spp, especially S. Typhi, from food, animal feedstuffs and environmental samples.
This product is relevant to food safety and environmental monitoring programs where Salmonella screening is part of raw material, finished product, or hygiene verification testing.
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When this format is useful
This product fits screening programs where a negative or presumptive result can affect raw-material release, finished-product hold decisions, or hygiene investigations. The lab should connect the medium to its enrichment sequence, confirmation method, and written rule for when suspect colonies move forward.
What to read after incubation
Salmonella Typhi appears as black colonies surrounded by a metallic sheen. Other Salmonella species have variable colonies which can be black, green, clear or mucoid. Other organisms, such as coliforms, Serratia and Proteus spp, are usually inhibited but occasionally there can be strains which give dull, green or brown colonies, with no metallic sheen.
Selective or enrichment logic
Meat extract, peptone and tryptone provide amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Glucose is the fermentable carbohydrate. Disodium phosphate is the buffering agent. Ferrous sulphate is an indicator of hydrogen sulfide production. Bismuth sulfite and brilliant green are selective agents effective against both Gram-positive and Gram-negative organisms other than Salmonella spp and some Shigella spp. Agar is the solidifying agent.
Sample handling workflow
- Preparation: NOTE: Prepare plates on day before streaking and store in dark. Selectivity may decrease after 48h.
- Procedure: Bismuth-Sulphite Agar may be used in conjunction with other selective agars for the isolation of salmonellae. Streak a loopful of the sample onto the surface of the agar. Incubate at 35 ± 2°C for 18-48 hours. Atypical colonies may appear if the medium is heavily inoculated with organic matter. To prevent such situation, suspend the sample in sterile saline and use the supernatant for inoculation. When the number of salmonellae is expected to be small, enrichments methods may be employed.
Technical verification points
- Formula / composition: * (g/litre) *Adjusted and/or supplemented as required to meet
Storage, hazards, and disposal
- Warnings / precautions: For professional use only. Operators must be trained and have certain experience in the laboratory methods. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store prepared plates at 2-8°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be sp ecially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610301
- Catalog/document code matched: 610301, 2022
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/bismuth-sulphite-agar-for-microbiology-lab-testing