Liofilchem Lauryl Sulfate Tryptose Broth Modified (m-LST) ISO 22964 Culture Media 610247 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is modified Lauryl Sulfate Tryptose Broth (mLST) is a medium for the selective enrichment of Cronobacter sakazakii in infant milk powder and milk based products. This medium is formulated according to ISO 22964.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Where this product fits
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Result signals and confirmation
Take a 10 µl loop from turbid tubes, streak onto the surface of the Enterobacter (Cronobacter) sakazakii Isolation Agar plate (ref. 11623). Incubate the plates at 44°C for 18-24 hours. Examine for the presence of typical colonies of Cronobacter sakazakii, which are small to medium sized, green to blue-green coloured.
Method principle
Enzymatic Digest of Casein and Animal Tissue provides nitrogen, vitamins, minerals and amino acids essential for organisms growth. Lactose is the fermentable carbohydrate. Potassium phosphates are the buffering agents. Sodium chloride in addition to supply essential electrolytes for the osmotic balance, at a so high concentration restricts the growth of other members of the Enterobacteriaceae. Also sodium lauryl sulfate acts as selective agent inhibiting organisms other than coliforms. Vancomycin is added to the medium to inhibit Gram-positive bacteria.
Bench workflow
- Preparation: Suspend 64.6 g of powder in 1 liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Aseptically, add the rehydrated content of 2 vials (10 ml) of Vancomycin (5 mg) Supplement (ref. 81064) to get 10 mg/l vancomycin in the complete medium. Dispense into 10 ml tubes.
- Procedure: Homogenise the sample diluted 1:10 in Buffered Peptone Water (ref. 24199) and incubate at 37C for 16-20 hours. Transfer 0.1 ml of thebroth culture into a tube of Modified Lauryl Sulfate Tryptose Broth (mLST). Incubate at 44°C for 24 hours.
Controls and performance checks
- Formula / composition: (g/l)Enzymatic Digest of Casein and Animal Tissue20.00Lactose 5.00Dipotassium Phosphate 2.75Monopotassium Phosphate 2.75Sodium Chloride34.00Sodium Lauryl Sulfate 0.10Final pH 6.8 ± 0.2
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: ≤100 CFUInoculum for selectivity: >103 CFUIncubation Conditions: 18-24 h at 44 ± 0.5°CMicroorganismGrowthCronobacter sakazakiiATCC 29544GoodEnterococcus faecalisATCC 29212InhibitedEscherichia coliATCC 25922GoodEscherichia coliATCC 8739GoodStaphylococcus aureusATCC 25923InhibitedTABLE OF SYMBOLS Batch code Do not reuse Manufacturer Use by Fragile, handle withcare Catalogue number Temperature limitation Contains sufficientfor <n> tests Caution, consult instructions for use LIOFILCHEM
Storage, limits, and safety
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for professional use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.REFERENCES1.ISO 22964: 2006. Milk and milk products detection of Enterobacter sakazakii.2.Guillame-Gentil O., Sonnard V., Kandahai M.C., Mauragg J.D., and Jootsen H.A. (2005) A simple Rapid Cultural Method for Detection of Enterobacter sakazakii in environmental samples. J Food Prot. 68,1:64-69.3.ISO 6887-1: 1999. Microbiology of food and animal feeing stuffs −
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The produ ct must be sp ecially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610247
- Catalog/document code matched: 22964, 610247
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/lauryl-sulfate-tryptose-broth-modified-m-lst-iso-22964-for-microbiology-lab-testing