Liofilchem ANTIBIOTIC TEST - Device for the evaluation of residual antimicrobial power in 80350 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is aNTIBIOTIC TEST is a device constituted by particular paper discs impregnated with living spores of Bacillus subtilis ATCC 6633 and with a dry culture medium. This device is indicated for the evaluation of residual antimicrobial power in urines, milk and other biological fluids. KIT CONTENTEach kit contains:•2 cartridges with 25 discs each, impregnated with living spores of Bacillus subtilis ATCC 6633 and with a dry culture medium;•50 plastic tubes.
This product is most relevant to urine culture workflows, where colony differentiation, count interpretation, and suppression of swarming organisms help the lab move from a mixed specimen to a useful diagnostic result.
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When this format is useful
In susceptibility testing, the consumable is only useful when the inoculum, medium, incubation, reading endpoint, and interpretive system are controlled together. The lab should treat the result as a method output, not as a standalone product property.
What to read after incubation
OF THE RESULTSThe development of a red color in the tube of the sample indicates the absence of antimicrobial substances or their presence at a lower concentration than sensitive limit of the methods.The absence of a red colour in the tube indicates the presence of antimicrobial substances in the sample at a higher concentration than sensitive limit of the method.
Selective or enrichment logic
OF THE METHODThe paper disk contains living spores of Bacillus subtilis ATCC 6633 and a culture medium with triphenyl- tetrazolium- chloride (TTC). Spores and mediumare released into solution after the addition of the liquid sample to examine (urine, milk or other biological fluid). Substances endowed with antimicrobial activity, eventually present in the liquid sample, inhibit Bacillus subtilis spores development, so that liquid in the tube does not change its colour. In the caseof lack of substances with antimicrobial activity, Bacillus subtilis spores sprout.
Sample handling workflow
- Procedure: 1.Take the cartridge containing the disks from the refrigerator and allow it to reach room temperature. 2.Take a disk of Antibiotic Test from the cartridge and put it into a tube.3.Add 0.2 mL of urine, milk or other biological fluid to test.4.Cap the tube and incubate at 36 ± 1 °C for 24 hours.5.Observe for development of a red colour after 8-12-24 hours.
Technical verification points
- Quality control: Each batch of ANTIBIOTIC TEST is submitted to
Storage, hazards, and disposal
- Warnings / precautions: Results obtained with ANTIBIOTIC TEST are qualitative.
- Storage / shelf life: OF THE SAMPLESIn order to perform the test on urine use the same sample of the urinoculture. For collection of milk or other biological fluids, follow the instructions suggested for culture tests on these samples.Store the samples at 2-8 °C before use.
- Waste disposal: OF USED MATERIALAfter use ANTIBIOTIC TEST and the materials come into contact with the sample have to be decontaminated and disposed of in accordance with the techniques used in laboratory for decontamination and
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product The lyophilized microorganisms and subsequent growt h of these microorganisms on culture media are cons idered to be biohazard material. Agencies and statutes regulate the
Procurement checklist
- Kormay SKU: KB7480350
- Catalog/document code matched: 80350, 6633
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/antibiotic-test-microbiology-testing-disc-lab-use-only-for-microbiology-lab-testing