Liofilchem MIDDLEBROOK 7H11 Agar Base Culture Media 610213 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is middlebrook 7H11 Agar Base is a medium used with supplements for the selective isolation of Mycobacterium spp, especially Mycobacterium tuberculosis, from clinical specimens as well as for the cultivation of pure cultures of mycobacteria. This agar-based medium provides improved susceptibility testing compared to media based on coagulated egg (Lowenstein formulations) where drugs may be more easily inactivated.
This product fits laboratories that culture or support workups for mycobacteria, where slow growth, selective recovery, incubation duration, and contamination control all affect the usefulness of the final result.
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Application fit for the lab
Mycobacteria workflows need a medium choice that accounts for slow growth, long incubation, contamination pressure, and careful colony review. The method should define how often plates are examined, what colony features matter, and which follow-up identification steps are required.
Expected colonies or reaction pattern
Examine weekly for growth, pigment production and colony morphology. Carry out identification tests according to established laboratory procedures. N.B. Negative culture results do not rule-out active infection by mycobacteria. Some factors that may lead to unsuccessful cultures are: •The specimens was not representative of the infectious materials; i.e. saliva instead of sputum. •The mycobacteria were destroyed during digestion and decontamination of the specimen. •Gross contamination interfered with the growth of mycobacteria. •Proper aerobic conditions and increased CO2 tension were not provided during incubation.
Principle behind the medium
Pancreatic digest of casein provides nitrogen, vitamins and amino acids for the growth of fastidious strains of M. tuberculosis. Glutamic acid, sodium citrate, pyridoxine, biotin and ammonium sulfate supply growth factors. Ferric ammonium citrate and magnesium sulfate are sources of trace ions. Phosphates help maintaining the pH of the medium. Malachite green is the selective agent inhibiting the contaminant microbial flora. Malachite green serves as pH indicator as well. Agar is the solidifying agent. Middlebrook 7H11 Agar Base is enriched with Glycerol Supplement (ref. 80021) and Middlebrook 7H10 (OADC) Supplement (ref. 81035) containing oleic acid, albumin, glucose, catalase and sodium chloride. Glycerol and glucose are energy sources. Sodium chloride maintains the osmotic equilibrium.
How to build it into the SOP
- Preparation: Suspend 20.5 g of powder in 900 millilitres of deionized or distilled water containing 5 ml of glycerol. Bring to boil and shake until completely dissolved. Sterilize at 121°C for 15 minutes. Cool up to 45-50°C. Aseptically, add 100 ml of Middlebrook 7H10 (OADC) Supplement. Mix well.
- Procedure: Inoculate the medium according to test procedures recommended by the Centers for Disease Control and Prevention (CDC) or consult appropriate
Composition and QC checkpoints
- Formula / composition: (g/l)Pancreatic Digest of Casein 1.0L-Glutamic Acid 0.5Sodium Citrate 0.4Pyridoxine Hydrochloride 0.001Biotin 0.0005Ferric Ammonium Citrate 0.04Ammonium Sulfate 0.5Disodium Phosphate 1.5Monopotassium Phosphate 1.5Magnesium Sulfate 0.05Malachite Green 0.001Agar15.0Final pH 6.6 ± 0.2 at 25°C
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74610213
- Catalog/document code matched: 610213, 80021, 81035
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/middlebrook-7h11-agar-base-for-microbiology-lab-testing