Liofilchem Sulfite indole motility agar (SIM agar) acc. to ISO 15213 Culture Media 610062 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is confirmation medium for enumeration of Clostridium perfringens, in food, feed, environmental samples and samples from the primary production stage. This medium is not intended for use in the diagnosis of disease or other conditions in humans.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
View this product on Kormay Biomedicals
When this format is useful
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
What to read after incubation
After incubation the tubes are examined for: - sulfite production: tubes showing blackening are positive; - motility: tubes showing growth outside the inoculation stab are positive; - indole production: tubes giving a red colored ring directly after adding Kovacs reagent are positive. C. perfringens is positive for sulfite production and negative for indole production and motility. © Liofilchem - SIM Agar ISO 15213-2 - Rev.1 / 27.11.2024 Page 2 of 4
Selective or enrichment logic
Peptone and enzymatic digest of casein provide amino acids, nitrogen, carbon, vitamins and minerals required for organisms growth. Ferrous ammonium sulfate and sodium thiosulfate serve as indicators for hydrogen sulfide (H2S) production through formation of a black precipitate. Agar is the solidifying agent. The low concentration of agar makes the medium semisolid allowing for visual determination of motility.
Sample handling workflow
- Preparation: or handling of food. SIM (Sulfite Indole Motility) Agar allows the differentiation of microorganisms and identification of C. perfringens on the basis of sulfite production, indole formation, and motility. It complies with the specification given by ISO 15213-2.
- Materials required: Standard microbiological supplies and equipment such as: Autoclave, Test tubes, inoculating loops, incubator,
- Procedure: Following the procedure outlined in ISO 15213-2, stab a SIM agar tube with colonies grown anaerobically on blood agar plates or nutrient agar plates. Incubate at 37 ± 1°C for 20-24 hours in an anaerobic atmosphere with loosen caps. Note: Test organisms must be in pure culture. The inoculum should be taken from a solid medium as inoculum from liquid suspensions may delay results. Erroneous results may occur if caps are not loose during incubation. For more details, consult appropriate guidance.
Technical verification points
- Formula / composition: * (Per Litre of Purified Water) Enzymatic Digest of Casein 6.0 g Enzymatic Digest of Soya 20.0 g Ferrous Ammonium Sulfate, anhydrous (FeH8N2O8S2) 0.2 g Sodium Thiosulfate (Na2O3S2) 0.2 g Agar 3.5 g Final pH 7.0 ± 0.2 at 25°C *Adjusted and/or supplemented as required to meet
Storage, hazards, and disposal
- Limitations: Invalid results can be caused by poor specimen quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the principles of the procedures, including its
- Warnings / precautions: For professional use only. Operators must be trained and have certain experience. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610062
- Catalog/document code matched: 15213, 610062, 2024
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/sulfite-indole-motility-agar-sim-agar-acc-to-iso-15213-for-microbiology-lab-testing