Application snapshot
Liofilchem CZAPEK DOX Agar Culture Media 610095 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is czapek Dox Agar is a medium used for the cultivation of fungi and bacteria capable of using inorganic nitrogen. Czapek Dox Agar is recommended by APHA for isolating Aspergillus, Penicillinum, Paecilomyces and related fungi, yielding characteristic mycelia and conidia. The only bacteria which are able to grow in this medium are non-fastidious soil bacteria.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Application fit for the lab
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Expected colonies or reaction pattern
Examine for microbial growth and chlamydospore production.
Principle behind the medium
Saccharose is the sole source of carbon and sodium nitrate is the sole nitrogen source. Dipotassium phosphate is the buffering agent. Magnesium sulfate, potassium chloride and ferrous sulfate provide essential ions. Agar is the solidifying agent.
How to build it into the SOP
- Preparation: Dehydrated medium Suspend 49 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Notice that the acidity of the medium may be increased to pH 3.5 ± 0.2 for the cultivation of acidophilic organisms, such as yeasts, by adding 10 ml of 10% lactic acid per liter after sterilization. Medium in bottles Melt the content of the bottle in a water bath at 100°C (loosing the cap partially removed) until completely dissolved.
- Procedure: Inoculate the medium by spreading the sample over the agar surface. Time and temperature of incubation vary considerably according to the species being cultivated. As a general guide, incubate for 1-2 weeks at 25°C. Most Penicillium spp have an optimum growth temperature between 20°C and 25°C, whilst many Aspergillus spp grow best at about 30°C. However, different fungi grow over a wide range of temperatures.
Composition and QC checkpoints
- Formula / composition: (g/l) Saccharose 30.0 Sodium Nitrate 2.0 Dipotassium Phosphate 1.0 Magnesium Sulfate 0.5 Potassium Chloride 0.5 Ferrous Sulfate 0.01 Agar 15.0 Final pH 7.3 ± 0.2 at 25°C
- Quality control: Plates are inoculated with the microbial strains indicated in the QC table. Inoculum for productivity: 50-100 CFU Incubation conditions: aerobically at 25-30°C for 48-72 hours.. QC Table. Microorganism Growth Aspergillus brasiliensis ATCC 16404 Good Candida albicans ATCC 10231 Good Saccharomyces cerevisiaeATCC 9763 Good WARNING AND
Handling limits and documentation
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for In vitro diagnostic use and must be used only by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store bottles and prepared plates at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration. © Liofilchem - Czapek Dox Agar - Rev.0 / 08.05.2015 Page 1 of 2 Instructions For Use
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74610095
- Catalog/document code matched: 610095, 2015
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/czapek-dox-agar-for-microbiology-lab-testing