Product overview
Liofilchem M.S.R.V. Medium Base Culture Media 610018 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is modified semi-solid Rappaport-Vassiliadis (MSRV) Medium Base is used with Novobiocin for the selective enrichment of motile Salmonellae in animal faeces and environmental samples. The medium meets the specifications for formulation and
This product is relevant to food safety and environmental monitoring programs where Salmonella screening is part of raw material, finished product, or hygiene verification testing.
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Where this product fits
This product fits screening programs where a negative or presumptive result can affect raw-material release, finished-product hold decisions, or hygiene investigations. The lab should connect the medium to its enrichment sequence, confirmation method, and written rule for when suspect colonies move forward.
Result signals and confirmation
A grey-white turbid zone extending out from the inoculated drop indicates a positive result for motile Salmonella spp. Negative plates, where the medium remains blue-green around inoculation drops, should be re-incubated for a further 18-27 h.Subculture should be carried out from the positive plates, with the inoculum being taken from the furthest edge of the migration zone. Presumptive identification is achieved by subculture onto XLD Agar (ref. 10056) and a second Salmonella agar of choice such as ChromaticTM Salmonella (ref. 11614).
Method principle
Enzymatic digest of animal and plant tissue and acid hydrolysate of casein provide amino acids, nitrogen, carbon, vitamins and minerals. Sodium chloride maintains the osmotic balance of the medium. Potassium dihydrogenphosphate is the buffer. Magnesium chloride raises the osmotic pressure. Malachite green oxalate inhibits organisms other than Salmonella spp. Novobiocin is added as a selective agent active mostly against Gram-positive bacteria. Agar is the solidifying agent.
Bench workflow
- Preparation: Suspend 31.6 g of powder in 1 liter of deionized or distilled water. Heat with frequent agitation and boil for 1 minute to completely dissolved the powder. DO NOT AUTOCLAVE. Cool up to 45-50°C. Aseptically, add the contents of 1 vial of Novobiocin Supplement (ref. 81021) reconstituted with 5 ml sterile distilled water. Mix well. Pour in Petri dishes.
- Procedure: For pre-enrichment, add the sample to Buffered Peptone Water (ref. 414020) at a ratio of 1:9 (e.g. 25 g per 225 ml), homogenize well and incubate at 36 ± 2°C for 16-20 h.Inoculate the MSRV Medium plates with 0.1 ml of the pre-enrichment culture (inoculate 3 drops in three different spots, equally spaced on the medium surface). Incubate at 41.5 ± 1°C for 18-27 h.
Controls and performance checks
- Formula / composition: (g/l)Enzymatic Digest of Animal and Plant Tissue 4.6Acid Hydrolysate of Casein 4.6Sodium Chloride 7.3Potassium Dihydrogenphosphate 1.5Magnesium Chloride anhydrous10.9Malachite Green Oxalate 0.04Agar 2.7Final pH 5.2 ± 0.1 at 25°C
- Performance: recommended by ISO 6579 Amendment 1.
Storage, limits, and safety
- Warnings / precautions: For professional use only. Operators must be trained and have certain experience in the laboratory methods. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document.Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.REFERENCES1.ISO 6579-1:2017+Amd1:2020. Microbiology of the food chain – Horizontal method for the detection, enumeration and serotyping ofSalmonella. Part 1: Detection of Salmonella spp.2.EN ISO 11133:2014+Amd1:2018. Microbiology of food, animal feed and water −
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be sp ecially treated adhering to official regulations administrative. Offer surp lus and non -recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610018
- Catalog/document code matched: 610018, 6579
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/m-s-r-v-medium-base-for-microbiology-lab-testing