Product and use case
Liofilchem ISOSENSITest Agar Culture Media 611265 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is isoSensitest Agar is a medium recommended for antimicrobial susceptibility testing of clinical isolates by the disc diffusion
This product belongs in antimicrobial or antifungal susceptibility testing, where the value is not the disc or strip alone but the controlled relationship between organism identity, inoculum, medium, incubation, and interpretation.
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When this format is useful
In susceptibility testing, the consumable is only useful when the inoculum, medium, incubation, reading endpoint, and interpretive system are controlled together. The lab should treat the result as a method output, not as a standalone product property.
What to read after incubation
After incubation measure the diameter of the zone of complete inhibition including the diameter of the disc. Interpret the size of the zones of inhibition by referring to the current BSAC breakpoints and report the organism as susceptible, intermediate or resistant to the agents that have been tested.
Selective or enrichment logic
The medium allows the growth of the great majority of microorganisms without further supplementation and the ingredients have been carefully selected to yield sharp and reproducible inhibition zones. The Kirby-Bauer method is based on the diffusion, through the agar, of the antimicrobial substance which soaks the paper disc. Each disc has a single concentration of the antimicrobial agent that inhibits the microorganism growth showing an halo around the disc. The diameter of the inhibiting halo is correlated with the Minimal Inhibitory Concentration (MIC).
Sample handling workflow
- Preparation: Dehydrated medium Suspend 31.4 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50°C. Aseptically dispense in Petri dishes. Medium in bottles Melt the content of the bottle in a water bath at 100°C (loosing the cap partially removed) until completely dissolved. Then screw the cap and check the homogeneity of the dissolved medium, if it is the case turning the bottle upside down. Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into Petri dishes.
- Procedure: 1.Prepare a standardized suspension of the test organism using either the direct colony suspension or growth method. 2.Dip a sterile cotton swab into the adjusted suspension. 3.Inoculate the surface of the plate by streaking the swab over the entire agar surface. 4.Dispense antimicrobial discs onto the surface of the inoculated agar plate. 5.Incubate aerobically at 35±2°C for 18-20 hours.
Technical verification points
- Formula / composition: (g/l) Hydrolysed Casein 11.0 Menadione (Vitamin K3) 0.001 Peptones 3.0 Cyanocobalamine (Vitamin B12) 0.001 Glucose 2.0 L-Cystein 0.02 Sodium Chloride 3.0 L-Tryptophan 0.02 Starch 1.0 Pyridoxin (Vitamin B6) 0.003 Disodium Hydrogen Phosphate 2.0 Pantothenate 0.003 Sodium Acetate 1.0 Nicotinamide 0.003 Magnesium Glycerophosphate 0.2 Biotin 0.0003 Calcium Gluconate 0.1 Thiamine 0.0004 Cobaltous Sulphate 0.001 Adenine 0.01 Cupric Sulphate 0.001 Guanine 0.01 Zinc Sulphate 0.001 Xanthine 0.01 Ferrous Sulphate 0.001 Uracil 0.01 Manganous Chloride 0.002 Agar 8.0 Final pH 7.4 ± 0.2 at 25°C
- Quality control: Plates are inoculated with the microbial strains indicated in the QC table. Antibiotic discs are then promptly placed onto the agar surface. © Liofilchem - IsoSensitest Agar - Rev.0 / 27.02.2014 Page 1 of 2 ENGLISH Instruction For Use Inoculum: 0.5 Mc Farland. Incubation conditions: aerobically at 35±2°C for 18-20 h. QC Table.
Storage, hazards, and disposal
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for In vitro diagnostic use and must be used only by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store tubes and bottles at room temperature away from light. Store prepared plates at 2-8°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74611265
- Catalog/document code matched: 611265, 2014
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/isosensitest-agar-for-microbiology-lab-testing-1