Product and use case
Liofilchem Cooked Meat Medium Culture Media 610372 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is cooked Meat Medium is used for the cultivation and maintenance of anaerobic organisms, especially pathogenic clostridia, differentiating saccharolytic from proteolytic species. This medium permits growth from a small inoculum, important for clinical specimens. It is also recommended in standard methods for food testing for the enumeration and identification of Clostridium perfringens.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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When this format is useful
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
What to read after incubation
Turbidity of the medium indicates microbial growth. In the cultivation of clostridia, saccharolytic organisms usually produce acid and gas. Growth of proteolytic organisms is generally characterized by blackening and dissolution of the meat particles.
Selective or enrichment logic
Heart tissue granules and peptic digest of animal tissue provide amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Solid meat particles provide favorable growth conditions for anaerobes due to reducing action of sulfhyrdyl (-SH) groups of muscle protein. Sulfhydryl groups are more accessible in denatured proteins, therefore the meat particles are cooked for use in the medium. Dextrose is the fermentable carbohydrate. Sodium chloride maintains the osmotic balance of the medium.
Sample handling workflow
- Preparation: Dehydrated medium Place 1.25 g of the particles in a test tube and add 10 milliliters of distilled or deionized water. Sterilize in autoclave at 121°C for 15 minutes. Medium in tubes To obtain proper incubation conditions for growth of strict anaerobes, Cooked Meat Medium should be reduced prior to use by boiling the tubes for 10 min with caps loosened and cooling with tightened caps to room temperature before inoculation. Alternatively, an efficient and easy way to obtain suitable anaerobic conditions is through the use of the GasPak EZ anaerobic system (ref. 96726, 96724 or 96728).
- Procedure: Inoculate the tube in the area of meat particles (bottom of the tube) with isolate from a fresh subculture medium. Incubate anaerobically at 35 ± 2°C for up to 7 days.
Technical verification points
- Formula / composition: (g/l) Heart Tissue Granules 98.0 Peptic Digest of Animal Tissue 20.0 Dextrose 2.0 Sodium Chloride 5.0 Final pH 7.2 ± 0.2 at 25°C
- Quality control: Tubes are inoculated with the microbial strains indicated in the QC table. Inoculum for productivity: 102-103 CFU. Incubation conditions: anaerobically at 35 ± 2°C for 40-48 hours. QC Table. Microorganism Growth Clostridium perfringens ATCC 12924 Good Clostidium sporogenes ATCC 11437 Good Staphylococcus aureus ATCC 25923 Good WARNING AND
Storage, hazards, and disposal
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for In vitro diagnostic use and must be used only by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store tubes at 2-8°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610372
- Catalog/document code matched: 610372, 2015
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/cooked-meat-medium-for-microbiology-lab-testing