Laboratory use profile
Liofilchem Legionella BCYE Agar Base Culture Media 610049 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is legionella BCYE Agar Base is a medium used with supplements for isolation and confirmation of Legionella colonies from clinical specimens and environmental samples.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Use case and sample types
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Decision criteria after incubation
Inspect plates for the first time either on day 2, 3, 4 or 5 followed by a final inspection at the end of the incubation period. Examine for growth and fluorescence under long-wave UV light. Colonies of Legionella are white-grey in general but can also appear in other colours. They are smooth with an entire edge and exhibit acharacteristic ground-glass
Method logic
Yeast extract provides amino acids, nitrogen, carbon, vitamins and minerals. Activated charcoal decomposes hydrogen peroxide, a metabolic product toxic to Legionella spp, and may also collect carbon dioxide and modify surface tension. Alpha-ketoglutarate stimulates organism growth. ACES buffer and potassium hydroxide are included to maintain the proper pH for optimal growth. Agar is the solidifying agent. This medium is supplemented with Legionella Growth Supplement (ref. 80056) to give Buffered Charcoal Yeast Extract (BCYE) Agar.To raise selectivity, one of the following supplements can be further added: •Legionella AB Supplement (ref. 81099) containing polymyxin B, cefazolin and pimaricin•Legionella GVPC Supplement (ref. 81008) containing vancomycin, polymyxin B and cycloheximide •Legionella MWY Supplement (ref.
Preparation and execution notes
- Preparation: Suspend 3.9 g of powder in 90 ml of deionized or distilled sterile water. Heat to boiling until completely dissolved. Sterlize in autoclave at121°C for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial (10 ml) of Legionella Growth Supplement.
- Procedure: Inoculate clinical specimens from swab by rolling it over the agar surface in order to obtain isolated colonies. According to ISO 11731, the method and culture media to be used will depend on the characteristics of the sample. See the scheme on page 2. For more details refer to the ISO standard.To ensure detection, water samples may be concentrated by membrane filtration or, alternatively, by centrifugation (when the number oflegionellae in any given sample is not known, concentration
Formula, QC, and performance
- Formula / composition: (g/l)Yeast Extract10.0Activated Charcoal 2.0Alpha-Ketoglutarate 1.0ACES Buffer10.0Potassium Hydroxide 2.8Agar13.0aFinal pH 6.9 ± 0.2 at 25°Ca Adjusted to provide adequate gelling strength.
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: 50-100 CFUInoculum for selectivity: 104-106 CFUIncubation conditions: 2-5 days at 36 ± 2°CMicroorganismGrowth on GVPC agar,BCYE+AB agar, MWY agarLegionella pneumophilaWDCM 00107GoodEnterococcus faecalisWDCM 00087InhibitedEscherichia coliWDCM 00013Partially to totally inhibitedTABLE OF SYMBOLS Batch code In vitro DiagnosticMedical Device Manufacturer Use by Fragile, handle with care Catalogue number Temperature limitation Contains sufficientfor <n> tests Consult instructionfor use Keep away
- Performance: testing of culture media. 3.Edelstein P.H. (1981) Improved semiselective medium for the isolation of Legionella pneumoniae from contaminated clinical and environmental specimens. J. Clin. Microbiol. 14(3):298. LIOFILCHEM S.r.l.Via Scozia, 64026, Roseto degli Abruzzi (TE) - ITALYTel +39 0858930745 Fax +39 0858930330 www.liofilchem.com liofilchem@liofilchem.com TECHNICAL SHEETTS 610049Rev.3.1 / 17.11.2021Page 3 of 3PRODUCT SPECIFICATIONSNAMELegionella BCYE Agar BasePRESENTATIONDehydrated medium
Risk controls for routine use
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for in vitro diagnostic use only and must be used by properly trained operators.
- Storage / shelf life: 10-30°C away from light, until the expiry date on the label or until signs of deterioration or contamination are evident.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to national and local regulations in force.LIOFILCHEM S.r.l.Via Scozia, 64026, Roseto degli Abruzzi (TE) - ITALYTel +39 0858930745 Fax +39 0858930330 www.liofilchem.com liofilchem@liofilchem.com TECHNICAL SHEETTS 610049Rev.3.1 / 17.11.2021Page 2 of 3Samples with an expectedDetection methodshigh level of Legionella spp (>104 cfu/l) and low level of contaminant organismsDirect plating•Spread 0.1 to 0.5 ml of the sample on one plate of BCYE agar and one plate of BCYE+AB agar low level of Legionella spp and low level of contaminant
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Skin Corr. 1A, Skin corrosion (Category 1A); H314 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Danger Hazard statement(s) H314 Causes severe skin burns and eye damage. Precautionary statement(s) P280 P303 + P361 + P353 P305 + P351 + P338 P310 P363 Wear protective gloves/protective clothing/eye protection/face protection.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610049
- Catalog/document code matched: 610049, 11731
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/legionella-bcye-agar-base-for-microbiology-lab-testing