Product and use case
Liofilchem Simmons CITRATE Agar Culture Media 610046 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is simmons Citrate Agar is used for the differentiation of Gram-negative enteric bacilli on the basis of citrate utilization. This medium is intended as an aid in the diagnosis, requiring additional tests to complete the diagnostic results.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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When this format is useful
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
What to read after incubation
A positive reaction is indicated by growth on the slant with an intense blue color (alkaline reaction). A negative reaction is shown by inhibition to poor growth without change in color, with the medium remaining green.
Selective or enrichment logic
Ammonium dihydrogen phosphate provides nitrogen as a sole source. Dipotassium phosphate acts as a buffer. Sodium chloride maintains the osmotic balance of the medium. Sodium citrate is the sole source of carbon in this medium. Magnesium sulfate is a cofactor for a variety of metabolic reactions. Bromthymol blue is the pH indicator. Agar is the solidifying agent. Organisms that can utilize ammonium dihydrogen phosphate and sodium citrate as the sole source of nitrogen and carbon, respectively, will grow on this medium and produce a color change from green (neutral) to blue (alkaline).
Sample handling workflow
- Preparation: Dehydrated medium Suspend 24.3 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil for 1 minute shaking frequently until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Medium in bottles Melt the content of the bottle in a water bath at 100°C (losing the cap partially removed) until completely dissolved. Then screw the cap and check the homogeneity of the dissolved medium, if it is the case turning the bottle upside down. Cool at 45-50°C, mix well avoiding foam formation and allow medium to solidify in a slanted position.
- Materials required: Standard microbiological supplies and equipment such as: Autoclave, water bath, sterile tubes, inoculating loops, swabs, incubator,
- Procedure: This medium is not intended to be used for primary isolation. Inoculate medium with growth from a pure culture. Streak only the surface of the slant with a light inoculum. © Liofilchem - Simmons Citrate Agar - Rev.0 / 06.03.2026 Page 2 of 4 Incubate tubes with loosened caps at 35 ± 2°C for 18-96 h in an aerobic atmosphere. Examine daily for growth and color change.
Technical verification points
- Formula / composition: * (g/litre) Ammonium Dihydrogen Phosphate 1.0 Dipotassium Phosphate 1.0 Sodium Chloride 5.0 Sodium Citrate 2.0 Magnesium Sulfate 0.2 Bromthymol Blue 0.08 Agar 15.0 Final pH 6.8 ± 0.2 at 25°C *Adjusted and/or supplemented as required to meet
- Quality control: organisms. SPECIMENS Clinical specimens should be sampled at the acute stage, before antimicrobial therapy (where possible) and examined as soon as possible after collection. Good laboratory practices for collection, transport and
Storage, hazards, and disposal
- Limitations: Invalid results can be caused by poor specimen quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the principles of the procedures, including its
- Warnings / precautions: 1) For in vitro diagnostic use (IVD). 2) For laboratory professional use only. 3) Operators must be trained and have certain experience. Please read the instructions carefully before using the product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. 4) Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. 5) Do not use if the product or packaging appears to be damaged. 6) Follow standard
- Storage / shelf life: of the clinical specimens should be applied. Refer to specific guidelines for more information about specimen collection and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610046
- Catalog/document code matched: 610046, 2026
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/simmons-citrate-agar-for-microbiology-lab-testing