Product overview
Liofilchem Asparagine ENRICHMENT Broth Culture Media 620138 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is aSPARAGINE ENRICHMENT BROTH is a selective medium, recommended by APHA, for Pseudomonas aeruginosa detection in pool and bathing waters.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Where this product fits
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Result signals and confirmation
free-flowing, homogeneous. Color: cream to white. Prepared medium
Bench workflow
- Procedure: Collect the specimen to be tested in bottles containing sodium thiosulfate (about 100mg / l of specimen) in order to neutralise the possible residual chlorine. Inoculate five containers with 10 ml, five with 1 ml and five with 0.1 ml of the sample into Asparagine Enrichment Broth. Incubate at 36 ± 1 °C for 48 hours. The presence of P. aeruginosa is shown by turbidity and a blue-green fluorescent pigment, observed with a long-wave ultraviolet light.
Controls and performance checks
- Formula / composition: (g/l) DL-Asparagine 3.0 Dipotassium Hydrogen Phosphate 1.0 Magnesium Sulphate 0.5 Final pH = 7.0 ± 0.2 at 25 °C. DIRECTIONS Suspend 4.5 g of powder in 1 liter of distilled or deionized water. Add 8 ml of glycerol, heat and shake until completely dissolved. Dispense into final containers. Sterilize in autoclave at 121 °C for 15 minutes.
Storage, limits, and safety
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared media at 2-8 °C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74620138
- Catalog/document code matched: 620138
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/asparagine-enrichment-broth-for-microbiology-lab-testing