Product and use case
Liofilchem ORNITHINE DECARBOXYLASE Broth Culture Media 610305 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is oRNITHINE DECARBOXYLASE BROTH is a dehydrated medium used in the biochemical differentiation of gram-negative enteric bacilli based on the production of ornithine decarboxylase.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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When this format is useful
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
What to read after incubation
A positive reaction is indicated by a purple color of the medium; a yellow color indicates a negative test.
Selective or enrichment logic
Peptone and meat extract supply the nitrogenous and other nutrients necessary to support bacterial growth. Pyridoxal is an enzyme co- factor for the ornithine decarboxylase. Dextrose is a fermentable carbohydrate. Bromocresol purple and cresol red are pH indicators. The ornithine is the amino acid to detect the production of ornithine decarboxylase. When the medium is inoculated with a bacterium that is able to ferment dextrose, acids are produced that lower the pH of the medium and change the colour of the indicator from purple to yellow. The acidic condition also stimulates decarboxylase activity. If the organism produces the ornithine decarboxylase, ornithine in the medium is degraded to putrescine. The production of this amine elevate the pH of the medium, changing the color of the indicator from yellow to purple.
Sample handling workflow
- Preparation: Suspend 21,0 g in 1 litre of distilled water. Heat until completely dissolved. Dispense into final containers. Autoclave at 121°C for 15 minutes.
- Procedure: Inoculate the broth media by transferring one or two colonies from the surface of a fresh culture with an inoculating loop and mix to distribute the culture throughout the medium. Overlay the medium in each tube with 1mL Vasellin Oil. Incubate at 36+/-1°C up to 96 hours.
Technical verification points
- Formula / composition: (g/L) Peptone............................................................................ 5.0 Meat Extract...................................................................... 5.0 Pyridoxal........................................................................... 0.005 Dextrose...………………………………………………....... 0.5 L-Ornithine...................................................................... 10.0 Bromocresol Purple......................................................... 0.010 Cresol Red........................................................................ 0.005 Final pH 6.0 ± 0.2
Storage, hazards, and disposal
- Warnings / precautions: The product is not classified as hazardous by current legislation. It is nevertheless recommended that the Safety Data Sheet be consulted on its correct use. The product is designed for In vitro diagnostic use and must be used only by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared media at 2-8°C. WARNING and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Eye Irrit. 2, Eye irritation (Category 2); H319 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H319 Causes serious eye irritation. Precautionary statement(s) P280 P305 + P351 + P338 P337 + P313 Wear protective gloves/protective clothing/eye protection/face protection.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610305
- Catalog/document code matched: 610305
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/ornithine-decarboxylase-broth-for-microbiology-lab-testing