Product and use case
Liofilchem KOSER CITRATE Medium Culture Media 610165 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is differential medium for presumptive Enterobacteria identification. This medium is intended as an aid in the identification tests to complete the diagnostic results.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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When this format is useful
Use this product where indicator organisms are part of a release, trend, or sanitation decision. The practical value comes from separating countable or presumptive target reactions from background flora so the analyst can document whether the sample meets the site limit.
What to read after incubation
Tubes with marked turbidity are considered positive for the Enterobacter group. E. coli will be inhibited showing no difference in comparison to a control tube (uninoculated). Note: further biochemical tests should be performed to confirm presumptive positive organisms identified with this medium.
Selective or enrichment logic
Sodium citrate allows growth of organisms such as Klebsiella aerogenes (Enterobacter aerogenes) and Enterobacter cloacae which are capable of utilizing citrate as the only source of carbon. Other organisms that cannot metabolise citrate, like Escherichia coli, will not grow in this medium. Sodium ammonium phosphate and magnesium sulphate provide nitrogen and magnesium ions, respectively. Monopotassium phosphate acts as a buffer.
Sample handling workflow
- Preparation: Dehydrated medium Suspend 5.7 g of the powder in 1 liter of distilled or deionized water. Mix well and heat shaking frequently until completely dissolved. Dispense into screw-cap tubes. Sterilize in autoclave at 121°C for 15 minutes.
- Materials required: Standard microbiological supplies and equipment such as: Autoclave, water bath, test tubes, inoculating loops, swabs, incubator,
- Procedure: Allow tubes to warm up to room temperature prior to inoculation. Inoculate the medium using a fresh (16-18 h) pure culture of the test organism. Incubate tubes at 35 ± 2°C for 18-24 h with caps slightly loosened.
Technical verification points
- Formula / composition: * (g/litre) *Adjusted and/or supplemented as required to meet
- Quality control: organisms. SPECIMENS Specimens should be obtained before antimicrobial therapy (where possible) and promptly delivered to the laboratory for examination. Refer to specific guidelines for more detailed information.
Storage, hazards, and disposal
- Limitations: Invalid results can be caused by poor specimen quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the principles of the procedures, including its
- Warnings / precautions: 1)For in vitro diagnostic use (IVD). 2)For laboratory professional use only. 3)Operators must be trained and have certain experience. Please read the instructions carefully before using the product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. 4)Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. 5)Do not use if the product or packaging appears to be damaged. 6)Follow standard
- Storage / shelf life: Instructions For Use Koser Citrate Medium For the differentiation of coliforms on the basis of citrate utilization. ENGLISH Sodium Citrate 3.0 Sodium Ammonium Phosphate 1.5 Potassium Phosphate (monobasic) 1.0 Magnesium Sulfate 0.2 Final pH 6.7 ± 0.2 at 25°C Page of 14 © Liofilchem - Koser Citrate Medium - Rev.0 / 14.10.2022 The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classif ication in any hazard class in accordance to Regula tion (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610165
- Catalog/document code matched: 610165, 2022
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/koser-citrate-medium-for-microbiology-lab-testing