Application snapshot
Liofilchem Azide Blood Agar Base Culture Media 610153 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is azide Blood Agar Base is a culture medium used for the detection and selective isolation of streptococci and staphylococci from stool, respiratory tract specimens, food and environmental samples with mixed flora. With added blood the medium may be used for the determination of haemolytic reactions. Other supplements may also be used for different purposes, e.g crystal violet for isolation of Erysipelothrix rhusiopathiae and Streptococcus pneumoniae.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Application fit for the lab
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Expected colonies or reaction pattern
Observe for growth and haemolytic reactions. Alpha-haemolysis: greenish discolouration of medium around the colony. Beta-haemolysis: clear zone around or under the colony. Gamma-haemolysis: no change. Azide Blood Agar Base Selective medium for detection of streptococci and staphylococci from clinical specimens and other materials. Instructions For Use ENGLISH Tryptospecial 18.0 Beef Extract 3.0 Sodium Chloride 5.0 Sodium Azide 0.2 Agar 15.0 Final pH 7.2± 0.2 at 25°C Dehydrated medium Suspend 41.2 g of the powder in 1 liter of distilled or deionized water. Mix well.
Principle behind the medium
Tryptospecial and beef extract provide amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Sodium chloride maintains the osmotic balance of the medium. Sodium azide acts largely inhibits the growth of gram-negative bacteria while sparing enterococci, staphylococci and streptococci. It also prevents the swarming of Proteus spp. Agar is the solidifying agent. The addition of blood serves as an additional source of growth factors and enables to differentiate microorganisms on the basis of haemolytic reactions. The haemolytic pattern or the degree of haemolysis may vary depending on the type of blood used and also if compared to the reactions on non-selective blood agar.
How to build it into the SOP
- Preparation: Cool to 45-50°C and aseptically add 5% horse or sheep blood. Mix well avoiding the formation of bubbles. Pour into Petri dishes. NOTE It is appropriate to use the medium freshly prepared. The prepared medium should be kept refrigerated for no longer than 48 hours.
- Procedure: For clinical use, inoculate by streaking the specimen onto the agar surface to obtain well isolated colonies. For food and environmental samples, spread 0.1 ml of the initial suspension and/or appropriate dilutions onto the surface of the medium. Incubate the inoculated plates inverted under appropriate atmosphere at 35 ± 2ºC for 18-24 h. Examine for growth and haemolytic reactions. If necessary, extend incubation for up to 40-48 h.
Composition and QC checkpoints
- Performance: specifications; Grams per litre of purified water.
Handling limits and documentation
- Warnings / precautions: For In Vitro Diagnostic use. For professional use only. Operators must be trained and have certain experience in the laboratory methods. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store bottles at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Acute Tox. 4, Acute toxicity, Oral (Category 4); H302 Aquatic Chronic 3, Chronic aquatic toxicity (Category 3); H412 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H302 H412 Harmful if swallowed. Harmful to aquatic life with long lasting effects.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Component N. CAS Value Control parameters Basis TWA 0,1 mg/m3 Indicative limit values for occupatio nal exposure to chemicals Sodium azide 26628-22-8 STEL 0,3 mg/m3 Indicative limit values for occupationa l exposure to chemicals 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74610153
- Catalog/document code matched: 610153, 2021
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/azide-blood-agar-base-for-microbiology-lab-testing