Application snapshot
Liofilchem Lactose GELATIN Medium Culture Media 610360 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is lACTOSE GELATIN MEDIUM is a dehydrated medium recommended for the identification of C. perfringens by lactose and gelatin testaccording to ISO 7937.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Application fit for the lab
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Expected colonies or reaction pattern
The isolated colonies which are not motile, reduce nitrate, ferment lactose and produce liquefaction of gelatine in 48 hours arepresumptively identified as Clostridium perfringens. (Note: not all Clostridium perfringens strains reduce nitrate to nitrite).
Principle behind the medium
Casamino acids is a hydrochloric acid hydrolysate of casein which supplies a completely hydrolyzed protein nitrogen source. Yeastextract provide nitrogen, vitamins, minerals and amino acids. Lactose is the fermentable carbohydrate, producing acid indicated by thephenol red indicator. Phenol red changes to yellow when acid is produced and to red when the medium becomes alkaline. Gelatin is aprotein derived by hydrolysis of collagen used to determine gelatinolysis by bacteria. The gelatinase produced by the microorganismshydrolyze the gelatin liquefying the solid medium.
How to build it into the SOP
- Preparation: Suspend 155 g of powder in 1000 ml of distilled water. Heat until completely dissolved. Dispense 10 ml portions into test tubes andsterilize in autoclave at 121°C for 15 minutes. If not used the same day, store the prepared medium at 2-8°C. Just before to use, heat inboiling water or flowing steam for 15 minutes, then cool rapidly to the incubation temperature.
- Procedure: Inoculate selected colony and incubate at 35 ± 2°C for 24-48 hours under anaerobic conditions. Examine the tubes for the presence ofgas and yellow colour (due to acid formation) indicating lactose fermentation. To read gelatinase, refrigerate until well chilled andcompare to non-inoculated tubes. Tubes positive for gelatinase will remain liquid.
Composition and QC checkpoints
- Formula / composition: (g/l)Casamino Acids 15.0Yeast Extract 10.0Lactose 10.0Phenol Red 0.05Gelatin120.0Final pH 7.5 ± 0.2 at 25°C
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: 10-100 CFU/mlInoculum for selectivity: 104-105 CFU/mlInoculum for specificity: ≤104 CFU/mlIncubation conditions: 24-48 h at 36 ± 1°CMicroorganismColor change to yellow and gas productionGelatinaseClostridium perfringensATCC 13124++Clostridium sporogenesATCC 25781-+TABLE OF SYMBOLS Batch code Keep away from heatsources Manufacturer Use by Catalogue number Temperature limitation Contains sufficient for<n> tests Consult instructionfor use LIOFILCHEM S.r.l.Via Scozia, Zona Ind.le - 64026,
Handling limits and documentation
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product must be usedonly by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use itbefore the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8°C away fromlight.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to national and local regulations in force.REFERENCES1.ISO 7937:2004. Microbiology of foods and animal feeding stuff – Horizontal method for the enumeration of C. perfringens. ColonyCount
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74610360
- Catalog/document code matched: 610360, 7937
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/lactose-gelatin-medium-for-microbiology-lab-testing