Application snapshot
Liofilchem MITIS SALIVARIUS Agar Culture Media 611020 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is mitis Salivarius Agar is a medium used with supplements for the selective isolation of viridans streptococci, such as Streptococcus mitis and Streptococcus salivarius, and enterococci, from specimens containing mixed microbial flora.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Application fit for the lab
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Expected colonies or reaction pattern
S. mitis cultivates with small blue colonies. These colonies may become easier to distinguish with longer incubation. S. salivarius produces blue, smooth or rough “gum drop” colonies, 1-5 mm in diameter depending on the number of colonies on the plate. Enterococcus spp. form dark blue or black, shiny, slightly raised, 1-2 mm colonies.
Principle behind the medium
Enzymatic digest of casein and enzymatic digest of animal tissue provide amino acids, nitrogen, carbon, minerals, vitamins and other nutrients for organisms growth. Sucrose and dextrose are the fermentable carbohydrates. Dipotassium phosphate is the buffering agent.Trypan blue is absorbed by the colonies, producing a blue colour. Crystal violet inhibits most Gram-negative bacilli and Gram-positive bacteria except streptococci. Agar is the solidifying agent. Potassium Tellurite 1% Supplement (ref. 80022) is added to the medium to aid in suppressing the contaminant bacterial flora.
How to build it into the SOP
- Preparation: Suspend 90.0 g of powder in 1 liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Sterilize at 121°C for 15 minutes. Cool up to 45-50°C. Aseptically, add 1 ml of Potassium Tellurite 1% Supplement. Pour in Petri dishes.
- Procedure: Inoculate and streak the specimen as soon as possible after it is received in the laboratory. Incubate the plates at 35 ± 2°C for 24-48 hours in atmosphere enriched with 5% carbon dioxide. Include a nonselective agar plate (e.g., blood agar) to increase the chance of recovering organisms present in low numbers and to provide an indication of other organisms present in the specimen.
Composition and QC checkpoints
- Formula / composition: (g/l)Enzymatic Digest of Casein15.0Enzymatic Digest of Animal Tissue 5.0Sucrose50.0Dextrose 1.0Dipotassium Phosphate 4.0Trypan Blue 0.075Crystal Violet 0.0008Agar15.0Final pH 7.0 ± 0.2 at 25°C
Handling limits and documentation
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for in vitro diagnostic use only and must be used by properly trained operators.
- Storage / shelf life: AND TRANSPORT CONDITIONSThe powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classif ication in any hazard class in accordance to Regula tion (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non-recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74611020
- Catalog/document code matched: 611020
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/mitis-salivarius-agar-for-microbiology-lab-testing