Laboratory use profile
Liofilchem LAURYL SULPHATE Agar Culture Media 610503 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is lAURYL SULFATE AGAR is a selective medium used for the isolation and enumeration of Escherichia coli and other coliforms fromwater by the membrane filtration method.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
View this product on Kormay Biomedicals
Use case and sample types
Use this product where indicator organisms are part of a release, trend, or sanitation decision. The practical value comes from separating countable or presumptive target reactions from background flora so the analyst can document whether the sample meets the site limit.
Decision criteria after incubation
Observe and count the yellow colonies over yellow zones in the medium. Report the results as CFU/ml of filtered sample.
Method logic
Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is the source of vitamins,particularly of the B-group. Lactose is a fermentable carbohydrate energy source. Sodium lauryl sulphate acts as selective inhibitor ofsporulating aerobic bacteria while has no effect over coliforms which grow quickly and abundant from minute inocula. Phenol red is a pHindicator which turns from red to yellow when there is acid production from lactose. Agar is the solidifying agent.
Preparation and execution notes
- Preparation: Suspend 92.2 g of powder in 1 lier of distilled water. Mix well and heat until completely dissolved. Autoclave at 121°C for 15 minutes.Cool to 45-50°C. Mix thoroughly and dispense in petri dishes.
- Procedure: Membrane filtration method: filter a suitable volume of sample through a sterile membrane, then place membrane filter, inoculum sideup, on the agar surface in the Petri dish. Incubate inverted plates at 35 ± 2°C for 24-48 hours. Coliform enumeration and E. coli enumeration must be done in separate volume of samples. The volume to be filtered must be carefulselected to obtain 10-100 colonies on the membrane.
Formula, QC, and performance
- Formula / composition: (g/l)Casein Peptone40.0Yeast Extract 6.0Lactose30.0Sodium Lauryl Sulphate 1.0Phenol Red 0.2Agar15.0Final pH 7.4 ± 0.2 at 25°C
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: 10-100 CFU/mlInoculum for selectivity: 104-105 CFU/mlInoculum for specificity: ≤104 CFU/mlIncubation conditions:18-24 h at 36 ± 1°CMicroorganismATCCGrowthFeaturesEscherichia coli25922GoodOrange-Yellow Media. Yellow ColoniesSalmonella typhimurium14028GoodRed Media. Colorless ColoniesPseudomonas euruginosa27853GoodRed Media.
Risk controls for routine use
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product must be usedonly by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use itbefore the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8°C away fromlight.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to national and local regulations in force.REFERENCES1.APHA 1998 Standard Methods for the examination of water and wastewater, 20th edition. LIOFILCHEM S.r.l.Via Scozia, Zona Ind.le - 64026, Roseto degli Abruzzi (TE) - ITALYTel +39 0858930745 Fax +39 0858930330 Website: www.liofilchem.net E-mail: liofilchem@liofilchem.net TECHNICAL SHEETTS 610503Rev.0 / 17.10.2012Page 2 of 2PRODUCT SPECIFICATIONSNAMELAURYL SULFATE AGARPRESENTATIONDehydrated medium
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610503
- Catalog/document code matched: 610503
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/lauryl-sulphate-agar-for-microbiology-lab-testing