Product and use case
Liofilchem Biggy (NICKERSON) Agar Culture Media 610135 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is selective and differential medium for the detection and isolation of Candida spp. from clinical samples. This medium is intended as an aid in the diagnosis, requiring further tests to complete the diagnostic results.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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When this format is useful
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
What to read after incubation
After incubation, observe the color and the morphology of the colonies for presumptive identification according to the following table. Microorganism Typical Colony Candida albicans Smooth, circular or hemispherical brown-black colonies; no color diffusion into surrounding medium; no metallic sheen. Candida tropicalis Smooth, discrete, dark brown to black colonies (may have black-colored centers); slight mycelial fringe; metallic sheen.
Selective or enrichment logic
Yeast extract provide amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Glycine stimulates growth. Glucose is the fermentable carbohydrate providing carbon and energy. Candida spp., through a process of substrate reduction, produce sulfide and bismuth which combine to produce brown to black pigmented colonies and zones of dark precipitate in the medium surrounding colonies of some species. Agar is the solidifying agent.
Sample handling workflow
- Preparation: Dehydrated medium Suspend 47.0 g of the powder in 1 liter of distilled or deionized water. Heat to boil shaking frequently until completely dissolved. DO NOT AUTOCLAVE. Medium in bottles Melt the content of the bottle in a water bath at 100°C (losing the cap partially removed) until completely dissolved. Then screw the cap and check the homogeneity of the dissolved medium, if it is the case turning the bottle upside down. Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into Petri dishes.
- Materials required: Standard microbiological supplies and equipment such as: water bath, sterile Petri plates, test tubes, inoculating loops, swabs, incubator,
- Procedure: Ensure there is no visible moisture on the plates before use. Inoculate the plates by directly streaking the specimen on the agar surface or spread the sample from an enrichment culture to obtain well-isolated colonies. Incubate plates in an inverted position (agar side up) aerobically at 22.5 ± 2.5°C for up to 5 days. © Liofilchem - Biggy (Nickerson) Agar - Rev.0 / 22.04.2024 Page 2 of 9 For more details, consult appropriate guidance.
Technical verification points
- Formula / composition: * (g/litre) Yeast Extract 1.0 Bismuth Ammonium Citrate 5.0 Sodium Sulphite 3.0 Glucose 10.0 Glycine 10.0 Agar 18.0 Final pH 6.8 ± 0.2 at 25°C *Adjusted and/or supplemented as required to meet
- Quality control: organisms. SPECIMENS Clinical specimens such as sputum, vaginal specimen, tissues, skin scrapings etc., should be sampled at the acute stage, before antimicrobial therapy (where possible) and examined as soon as possible after collection. Good laboratory practices for collection, transport and
Storage, hazards, and disposal
- Limitations: Invalid results can be caused by poor specimen quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the principles of the procedures, including its
- Warnings / precautions: 1) For in vitro diagnostic use (IVD). 2) For laboratory professional use only. 3) Operators must be trained and have certain experience. Please read the instructions carefully before using the product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. 4) Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. 5) Do not use if the product or packaging appears to be damaged. 6) Follow standard
- Storage / shelf life: of the clinical specimens should be applied. Refer to specific guidelines for more information about specimen collection and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610135
- Catalog/document code matched: 610135, 2024
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/biggy-nickerson-agar-for-microbiology-lab-testing