Product overview
Liofilchem DRBC Agar modified Culture Media 610399 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is dRBC (Dichloran Rose Bengal Chloramphenicol) Agar modified is a selective medium used for the enumeration of viable yeasts and moulds in products intended for human and animal consumption that have a water activity greater than 0.95.This medium complies with the requirements of ISO 21527-1.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Where this product fits
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Result signals and confirmation
Observe growth after 2 days and again after 5 days of incubation. Read the plates containing less than 150 colonies/propagules. Colonies of yeasts appear pink due to the uptake of rose bengal. For identification of yeast and moulds, perform high microscopic examination or inoculate on suitable media.
Method principle
Enzymatic digest of plant and animal tissues provides carbon, nitrogen, vitamins and minerals required for organism growth. Glucose is an energy source. Phosphate is a buffering agent. Magnesium sulfate is a source of divalent cations and sulfate. The antifungal agent, dichloran, is included to reduce colony diameters of spreading fungi. Rose bengal suppresses the growth of bacteria and restricts the size and height of colonies of the more rapidly growing moulds. Chloramphenicol and chlortetracycline are incorporated in the medium to inhibit the majority of bacteria present in environmental and food samples. Inhibition of bacterial growth and restriction of spreading ofmore-rapidly growing moulds aids in the isolation of slow-growing fungi. Also the low pH serves to prevent the growth of most bacteria.
Bench workflow
- Preparation: Suspend 31.64 g of the powder in 1 liter of purified water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Add 1 ml of Tergitol (ref. 80305). Autoclave at 121°C for 15 minutes. Cool to 45-50°C. Pour into Petri dishes.
- Procedure: Inoculate 0.1 ml of sample and/or its appropriate decimal dilutions by spreading over the entire agar surface. Incubate plates aerobicallyat 25 ± 1°C for 5 days.
Controls and performance checks
- Formula / composition: (g/l)Enzymatic Digest of Animal and Plant Tissues 5.0Glucose10.0Potassium Dihydrogenphosphate 1.0Magnesium Sulfate 0.5Dichloran (2,6-dichloro-4-nitroanile) 0.002Rose Bengal 0.025Chloramphenicol 0.050Chlortetracycline Chlorhydrate 0.050Zinc Sulfate 0.010Cupric Sulfate 0.005Agar15.0Final pH 5.6 ± 0.2 at 25°C
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: 50-100 CFUInoculum for selectivity: 104-106 CFU Incubation conditions: 5 days at 25 ± 1°CMicroorganismGrowthSaccharomices cerevisiaeWDCM 00058Good (PR ≥ 0.5)Aspergillus brasiliensisWDCM 00053Good (PR ≥ 0.5)Candida albicansWDCM 00054Good (PR ≥ 0.5)Escherichia coliWDCM 00012InhibitedBacillus subtilisWDCM 00003InhibitedTABLE OF SYMBOLS Batch code Keep away from heat sources Manufacturer Use by Fragile, handle with care Catalogue number Temperature limitation Contains sufficientfor <n> tests
- Performance: testing of culture media. 2.ISO 21527-1:2008. Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of yeasts and moulds3.Beuchat and Cousin. 2001. In Downes and Ito (ed.). Compendium of methods for the microbiological examination of foods,4th ed. American Public Health Association. Washington, D.C. 4.U.S. Food and Drug Administration. 1995. Bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, Md. 5.Banks, Board and Paton. 1985. Lett. Appl. Microbiol. 1:7.6.King, Hocking and Pitt. 1979. Appl. Environ. Microbiol. 37:959.
Storage, limits, and safety
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product must be used only by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to national and local regulations in force.REFERENCES1.EN ISO 11133:2014+Amd1:2018. Microbiology of food, animal feed and water −
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Carc. 1A, carcinogenicity(Category 1B); H350 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Danger Hazard statement(s) H350 May cause cancer Precautionary statement(s) P201 P281 P308+P313 Obtain special instructions before use. Use personal protective equipment as required.
- Exposure controls: 8.1 Control parameters Compone nts with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste tr eatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610399
- Catalog/document code matched: 610399, 21527
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/drbc-agar-modified-for-microbiology-lab-testing