Application snapshot
Liofilchem GIOLITTI CANTONI Broth Base Culture Media 620100 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is gIOLITTI-CANTONI BROTH BASE is used with Potassium Tellurite 3.5% supplement in enriching Staphylococcus aureus from foods during isolation procedures, as recommended by ISO 6888-3: 2003.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Application fit for the lab
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Expected colonies or reaction pattern
free-flowing, homogeneous. Color: tan. Prepared medium
How to build it into the SOP
- Procedure: Inoculate 1 g or 1 ml of test sample (0.1 g or 0.1 ml when testing meat or meat products) and 1 ml of each of a suitable decimal dilution series of the test sample into duplicate tubes. Overlay each tube with 5 ml of sterile molten paraffin wax to an approximate height of 2 cm. Incubate at 36 ± 1 °C for 40-48 hours. Examine daily. Read tubes for blackening of the medium (a positive reaction) or no blackening (a negative reaction). If blackening occurs subculture on Baird Parker Agar, it confirms the isolation of S. aureus.
Composition and QC checkpoints
- Formula / composition: (g/l) Tryptone 10.0 Beef Extract 5.0 Yeast Extract 5.0 D-Mannitol 20.0 Lithium Chloride 5.0 Sodium Chloride 5.0 Glycine 1.2 Sodium Pyruvate 3.0 Final pH = 6.9 ± 0.2 at 25 °C. DIRECTIONS Suspend 54.2 g of powder in 1 liter of distilled or deionized water. Heat gently and shake until completely dissolved. Distribute into tubes in amounts of 19 ml. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C. Aseptically add 0.3 ml of Potassium Tellurite 3.5% supplement (code 80291) to each tube or 0.03 ml when testing meat products or
Handling limits and documentation
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared tubes at 2-8 °C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74620100
- Catalog/document code matched: 620100
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/giolitti-cantoni-broth-base-for-microbiology-lab-testing