Liofilchem Dichloran Glycerol (DG 18) Agar Base w/Chloramphenicol Culture Media 610401 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is dichloran Glycerol (DG18) Agar Base w/Chloramphenicol is used with supplements for the selective isolation and enumeration of fungi in food, animal feed and environmental samples. The complete medium conforms to ISO 21527-2 for microbiological examination of products that have a water activity less than or equalto 0.95 such as dry fruits, cakes, jams, dried meat, salted fish, grains, cereal products, flour, nuts, spices and condiments, etc.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Where this product fits
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Result signals and confirmation
Select plates containing <150 colonies/propagules/germs and count after 2 days and again after 5-7 days of incubation. Report theresults as CFU per g or per ml of sample allowing for the dilution factor.
Method principle
Enzymatic digest of casein provides amino acids, carbon, nitrogen, vitamins and minerals for organisms growth. Glucose is included as energy source. Monopotassium phosphate is a buffering agent. Magnesium sulfate provides divalent cations and sulfur. Dichloran is an antifungal agent incorporated into the medium to reduce colony diameters of spreading fungi easing the colony count. Chloramphenicol inhibits the growth of accompanying bacterial flora. Inhibition of bacterial growth and restriction of spreading of more-rapidly growing moulds aids in the isolation of slow-growing fungi. Agar is the solidifying agent. Glycerol Supplement (ref. 80021) is included to lower the water activity of the medium and provide an additional carbon source.
Bench workflow
- Preparation: Suspend 31.6 g of powder in 1 liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Add 220 g glycerol (about 180 ml Glycerol Supplement). Sterilize at 121°C for 15 minutes. Cool up to 45-50°C. Pour in Petri dishes.
- Procedure: According to ISO 21527-2, spread 0.1 ml of the test sample or 0.1 ml of the initial suspension onto one plate of Dichloran Glycerol (DG18) Agar and 0.1 ml of the first decimal dilution over a second plate. Repeat inoculation with subsequent dilutions. Incubate inoculated plates aerobically at 25 ± 1°C for 5-7 days and up to 10 days if the presence of Xeromyces bisporus is suspected.
Controls and performance checks
- Formula / composition: (g/l)Casein Enzymatic Digest 5.0D-Glucose10.0Monopotassium Phosphate 1.0Magnesium Sulfate 0.5Dichloran 0.002Agar15.0Chloramphenicol 0.1Final pH 5.6 ± 0.2 at 25°C
- Performance: testing of culture media.2.ISO 21527-2:2008. Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of yeasts and moulds. Part 2: Colony Count
Storage, limits, and safety
- Warnings / precautions: For professional use only. Operators must be trained and have certain experience in the laboratory methods. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from theinstructions in this document.Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.REFERENCES1.EN ISO 11133:2014+Amd1:2018+Amd2:2020. Microbiology of food, animal feed and water −
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Carc. 1A, carcinogenicity (Category 1A); H350 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Danger Hazard statement(s) H350 May cause cancer Precautionary statement(s) P201 P281 P308+P313 Obtain special instructions before use. Use personal protective equipment as required.
- Exposure controls: 8.1 Control parameters Components with workpl ace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment me thods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610401
- Catalog/document code matched: 610401, 21527
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/dichloran-glycerol-dg-18-agar-base-w-chloramphenicol-for-microbiology-lab-testing