Application snapshot
Liofilchem Rappaport Vassiliadis Soy (RVS) Broth 10 ml Culture Media 620175 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is liquid medium for selective enrichment of Salmonella spp. from clinical specimens, food, animal feed, environmental specimens. This medium conforms to the requirements of ISO 6579-1. This medium is intended as an aid in the diagnosis, requiring additional tests to complete the diagnostic results.
This product is relevant to food safety and environmental monitoring programs where Salmonella screening is part of raw material, finished product, or hygiene verification testing.
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Application fit for the lab
This product fits screening programs where a negative or presumptive result can affect raw-material release, finished-product hold decisions, or hygiene investigations. The lab should connect the medium to its enrichment sequence, confirmation method, and written rule for when suspect colonies move forward.
Expected colonies or reaction pattern
The presence of turbidity compared to uninoculated control, or a pellicle formation indicates microbial growth. Subculture to suitable solid media for complete identification of the isolated colonies.
Principle behind the medium
Enzymatic digest of soya provides amino acids, nitrogen, carbon, minerals and vitamins for organisms growth. Sodium chloride maintains the osmotic balance of the medium. Potassium phosphates act as a buffer. Magnesium chloride and malachite green are the selective agents. The low pH helps inhibit non-target organisms.
How to build it into the SOP
- Preparation: Dehydrated medium Suspend 26.6 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. Dispense into final containers. Sterilize in autoclave at 115°C for 15 minutes.
- Materials required: Standard microbiological supplies and equipment such as: inoculating loops, sterile cotton swabs, physiological solution, incubator,
- Procedure: Following the method described in the ISO 6579-1, transfer 0.1 ml of the culture obtained in the pre-enrichment (Buffered Peptone Water, ref. 24099) to a tube containing 10 ml of the RVS Broth. Incubate at 41.5 ± 1°C for 24 ± 3 hours. Use the culture obtained to inoculate the selective solid media, i.e. XLD agar (ref. 10056) and a second isolation agar. For more information, see the ISO document. Notes: © Liofilchem - Rappaport Vassiliadis Soy (RVS) Broth - Rev.0 / 15.09.2025 Page 2 of 7 Before use, allow RVS broth to equilibrate at room temperature if it was stored at a lower temperature. For some products, like dried milk products and cheese, it may be necessary to incubate the selective enrichment medium for an additional 24 h. After incubation, the selective enrichment can be stored refrigerated at 5 ± 3°C for up to 72 h.
Composition and QC checkpoints
- Formula / composition: * (Per liter of Purified Water) Enzymatic Digest of Soya 4.5 g Sodium Chloride 7.2 g Potassium Dihydrogen Phosphate (KH2PO4) 1.26 g Dipotassium Hydrogen Phosphate (K2HPO4) 0.18 g Magnesium Chloride Anhydrous 13.4 g Malachite Green 0.036 g Final pH 5.2 ± 0.2 at 25°C *Adjusted and/or supplemented as required to meet
- Quality control: organisms. SPECIMENS Clinical specimens should be sampled at the acute stage, before antimicrobial therapy (where possible) and examined as soon as possible after collection. Good laboratory practices for collection, transport and
- Performance: specifications. Grams per liter of purified water
Handling limits and documentation
- Limitations: Invalid results can be caused by poor sample quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the principles of the procedures, including its
- Warnings / precautions: 1) For in vitro diagnostic use (IVD). 2) For laboratory professional use only. © Liofilchem - Rappaport Vassiliadis Soy (RVS) Broth - Rev.0 / 15.09.2025 Page 3 of 7 3) Operators must be trained and have certain experience. Please read the instructions carefully before using the product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. 4) Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. 5) Do not use if the product or packaging appears to be damaged. 6) Follow standard
- Storage / shelf life: of the clinical specimens should be applied. Refer to specific guidelines for more information about specimen collection and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74620175
- Catalog/document code matched: 620175, 2025, 6579
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/rappaport-vassiliadis-soy-rvs-broth-10-ml-for-microbiology-lab-testing