Product and use case
Liofilchem Buffered Listeria ENRICHMENT Broth Culture Media 610324 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is bUFFERED LISTERIA ENRICHMENT BROTH is a selective enrichment medium for the detection of Listeria spp. in food. The addition of potassium di-hydrogen orthophosphate and disodium hydrogen orthophosphate increase the buffering capacity of the medium.
This product supports Listeria workflows in ready-to-eat food, dairy, meat, and environmental monitoring programs where early presumptive recovery must lead into confirmation.
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When this format is useful
Use this product where the lab must protect a recovery step before confirmation. For ready-to-eat food and environmental samples, the important decision is not only whether growth appears, but whether the enrichment or plating step gives the organism enough opportunity to recover from stress, competing flora, preservatives, or cold-chain exposure.
What to read after incubation
free-flowing, homogeneous. Colour: Straw coloured. Prepared medium
Sample handling workflow
- Procedure: 1. Add 25 g or 25 mL samples to 225 mL of Buffered Listeria Enrichment Broth. Homogenise if required. 2. Incubate at 30°C for 48 hours. 3. Subculture from the Buffered Listeria Enrichment Broth onto Listeria Palcam Agar plates (code 10041) or Listeria Esculin Agar plates (code 11230) after 24 and 48 hours by: • Direct plating onto Listeria Palcam Agar plates or Listeria Esculin Agar plates. • Adding 1 mL of the Buffered Listeria Enrichment Broth to 9 mL of 0.5% KOH, vortex mixing, and plating onto Listeria Palcam Agar plates or Listeria Esculin Agar plates.
Technical verification points
- Formula / composition: (g/l) Tryptone soya broth 30.0 Yeast Extract 6.0 Potassium di-hydrogen orthophosphate 1.35 Disodium hydrogen orthophosphate 9.6 Final pH = 7.3 ± 0.2 at 25°C. DIRECTIONS Add 23.5 g of powder to 500 mL of distilled or deionized water and mix well to dissolve. Sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50°C and aseptically add the contents of 1 vial of MODIFIED LISTERIA SELECTIVE ENRICHMENT SUPPLEMENT (code 81044). Mix well and aseptically distribute into sterile containers in volumes as required.
Storage, hazards, and disposal
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10- 30°C, in a dry environment, in its original container tightly closed away from bright light. When stored as directed the medium will remain stable until the expiry date printed on the label. Store prepared containers at 2-8°C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The produ ct must be sp ecially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Procurement checklist
- Kormay SKU: KB74610324
- Catalog/document code matched: 610324, 1989
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/buffered-listeria-enrichment-broth-for-microbiology-lab-testing