Product overview
Liofilchem m-CP Agar Base Culture Media 610199 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is m-CP Agar Base is a selective medium used with supplements for isolating and enumerating Clostridium perfringens from water samples by the membrane filtration
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Where this product fits
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Result signals and confirmation
Clostridium perfringens ferments sucrose and does not ferment cellobiose (beta-D-glucosidase-negative) producing characteristic opaque yellow colonies. Furthermore, colonies turn pink or red after 20-30 sec exposure to ammonium hydroxide vapour due to acid phosphatase activity.Most other clostridia which cleave the chromogenic substrate indoxyl-beta-D-Glucoside will form either purple or blue-green colonies depending on the ability to ferment sucrose as well.For confirmation of C.
Method principle
Tryptose provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Yeast extract is a source of vitamins, particularly of B-group. Sucrose is the fermentable carbohydrate. L-Cysteine is the reducing agent. Bromocresol purple is the pH indicator. Agar is the solidifying agent.The medium must be supplied with the following supplements to inhibit the accompanying non clostridial flora and differentiate Clostridium perfringens from other Clostridium spp: •m-CP Supplement (D-Cycloserine and Polymixyn B);•m-CP Supplement A (Phenolphtalein diphosphate);•m-CP Supplement B (Ferric Chloride);•m-CP Supplement C (Indoxyl-beta-D-Glucoside).
Bench workflow
- Preparation: Suspend 35.5 g of powder in 500 millilitres of deionized or distilled water. Bring to boil and shake until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Cool up to 45-50°C. Aseptically, add the rehydrated content of the 4 vials indicated below: •1 vial (5 ml) of m-CP Supplement (ref. 81034);•1 vial (10 ml) of m-CP Supplement A (ref. 81070);•1 vial (1 ml) of m-CP Supplement B (ref. 81071);•1 vial (4 ml) of m-CP Supplement C (ref. 81072);Mix well. Pour in Petri dishes.
- Procedure: as recommended in the Directive of the Council of the European Union 98/83/EC.
Controls and performance checks
- Formula / composition: (g/l)Tryptose30.0Yeast Extract20.0Sucrose 5.0L-Cysteine Hydrochloride 1.0Magnesium Sulfate 0.1Bromocresol Purple 0.04Agar15.0Final pH 7.6 ± 0.2 at 25°C
Storage, limits, and safety
- Warnings / precautions: The product contains hazardous substances and is classified as dangerous. It is recommended to consult the safety data sheet for its correct use. The product is designed for professional use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610199
- Catalog/document code matched: 610199
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/m-cp-agar-base-for-microbiology-lab-testing