Application snapshot
Liofilchem Legionella CYE Agar Base Culture Media 610125 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is legionella CYE Agar Base is a medium used with supplements for confirmation of presumptive Legionella colonies from clinical specimens and environmental samples.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Application fit for the lab
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Expected colonies or reaction pattern
Examine for growth and fluorescence under long-wave UV light. Regard as Legionella those colonies which grow on Legionella BCYE Agar but fail to grow on the medium without cysteine. Colonies of Legionella are often white-grey-blue-purple in colour, but can be brown, pink, lime-green or deep-red. They are smooth with an entire edge and exhibit a characteristic ground-glass
Principle behind the medium
Yeast extract provides amino acids, nitrogen, carbon, vitamins and minerals. Activated charcoal decomposes hydrogen peroxide, a metabolic product toxic to Legionella spp, and may also collect carbon dioxide and modify surface tension. Agar is the solidifying agent This medium can be supplemented with one of the following:•Legionella BCYE Growth Supplement (ref. 81056) to give Legionella BCYE Agar;•Legionella BCYE Growth Supplement w/o L-Cysteine (ref. 81091) to prepare the medium as above but omitting L-cysteine.
How to build it into the SOP
- Preparation: Suspend 2.4 g of powder in 90 ml of deionized or distilled sterile water. Heat to boiling until completely dissolved. Sterlize in autoclave at121°C for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial (10 ml) of either Legionella BCYE Growth Supplement or Legionella BCYE Growth Supplement w/o L-Cysteine, as desired. Mix well. Dispense in petri dishes.
- Procedure: Inoculate clinical specimens from swab by rolling it over the agar surface in order to obtain isolated colonies. ISO 11731 recommends to subculture typical colonies from Legionella Agar (GVPC) plates (ref. 10128) onto plates of Legionella BCYE Agar and Legionella BCYE Agar without cysteine for confirmation of presumptive Legionella colonies. Incubate plates at 36 ± 2°C for at least 2 days in humidified atmosphere (air with 2.5% CO2 can be beneficial for the growth of some Legionella but is not essential).
Composition and QC checkpoints
- Formula / composition: (g/l)Yeast Extract10.0Activated Charcoal 2.0Agar12.0Final pH 6.9 ± 0.4 at 25°C
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlInoculum for productivity: 50-100 CFUInoculum for selectivity: 104-106 CFUIncubation conditions: 2-5 days at 36 ± 2°CMicroorganismGrowth on Legionella BCYE AgarGrowth on Legionella BCYE Agar w/o CysteineLegionella pneumophilaWDCM 00107GoodInhibitedLegionella bozemaniiNCTC 11368GoodInhibitedStaphylococcus aureusATCC 25923GoodGoodEscherichia coliATCC 25922GoodGoodTABLE OF SYMBOLS Batch code In vitro DiagnosticMedical Device Manufacturer Use by Fragile, handle with care Catalogue number Temperature limitation Contains
Handling limits and documentation
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for in vitro diagnostic use only and must be used by properly trained operators.
- Storage / shelf life: 10-30°C away from light, until the expiry date on the label or until signs of deterioration or contamination are evident.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to national and local regulations in force.REFERENCES1.ISO 11731-2:2004. Water quality − Detection and enumeration of Legionella. Part 2: Direct membrane filtration method for waters with low bacterial count.2.ISO 11731:1998. Water quality − Detection and enumeration of Legionella.3.Edelstein P.H. (1981) Improved semiselective medium for the isolation of Legionella pneumoniae from contaminated clinical and environmental specimens. J. Clin. Microbiol. 14(3):298.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74610125
- Catalog/document code matched: 610125, 11731
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/legionella-cye-agar-base-for-microbiology-lab-testing