Product overview
Liofilchem YERSINIA Selective Agar Base Culture Media 610111 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is yersinia Selective Agar Base is a medium used with supplements for the selective isolation and differentiation of Yersinia enterocolitica The complete medium (CIN agar) is recommended by ISO 10273 for the examination of food and animal feed stuffs as well as environmental samples in the area of food production and food handling.
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Where this product fits
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Result signals and confirmation
Organisms capable of fermenting mannitol cause a localized pH reduction, forming colonies with red centre surrounded by a transparentborder (characteristic “bull’s-eye” colony). Organisms that do not ferment mannitol form colorless, translucent colonies. Some strains ofSerratia,Citrobacter andEnterobacter may give a colonial morphology resemblingYersinia enterocolitica. Final identification should beconfirmed by standard biochemical tests.
Method principle
Enzymatic digest of gelatin and enzymatic digest of casein and animal tissues provide amino acids, nitrogen, carbon, minerals, vitamins and other nutrients which support the growth of microorganisms. Yeast extract is a source of vitamins, particularly of B-group. Sodium chloride maintains the osmotic balance of the medium. Sodium pyruvate and magnesium sulfate stimulate organisms growth. Mannitol is the carbohydrate which allows to differentiate between mannitol fermenting and non-fermenting bacteria. Sodium deoxycholate and crystal violet inhibit Gram-positive bacteria. Neutral red is the pH indicator. Agar is the solidifying agent. Supplementation with Yersinia Supplement (ref. 81039), containing cefsulodin, irgasan (triclosan) and novobiocin, inhibits the growth of most Gram-negative enteric bacteria.
Bench workflow
- Preparation: Suspend 59.6 g of powder in 1 liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Sterilize at 121°C for 15 minutes. Cool up to 45-50°C. Aseptically, add the contents of 2 vials (6 ml) of Yersinia Supplement reconstituted as directed in the instructions for use that accompany the product. Pour in Petri dishes.
- Procedure: Inoculate the specimen onto the medium by either direct plating or pour plating (*). Incubate aerobically at 30 ± 1°C for 18-24 h.(*) The ISO method for the detection of presumptive pathogenic Yersinia enterocolitica recommends to first perform enrichment in Peptone, Sorbitol and Bile Salts (PSB) Broth for 48-72 hours at 22-25°C with agitation, or 5 days without agitation.
Controls and performance checks
- Formula / composition: (g/l)Enzymatic Digest of Gelatin17.0Enzymatic Digest of Casein and Animal Tissues 3.0Yeast Extract 2.0Sodium Chloride 1.0Sodium Pyruvate 2.0Magnesium Sulfate 0.01Mannitol20.0Sodium Deoxycholate 0.5Crystal Violet 0.001Neutral Red 0.03Agar14.0Final pH 7.4 ± 0.2 at 25°C
Storage, limits, and safety
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. it is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for in vitro diagnostic use only and must be used by properly trained operators.
- Storage / shelf life: AND TRANSPORT CONDITIONSThe powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until sings of deterioration or contamination are evident. Store prepared plates at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610111
- Catalog/document code matched: 610111, 10273
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/yersinia-selective-agar-base-for-microbiology-lab-testing