Laboratory use profile
Liofilchem Sabouraud Dextrose Agar Dehydrated Culture Media 610103 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is medium for the cultivation and enumeration of yeasts and moulds from clinical and non-clinical specimens. This medium is intended as an aid in the diagnosis, requiring further tests to complete the diagnostic results.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Use case and sample types
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Decision criteria after incubation
Transfer of growth from slants to plated media may be required in order to obtain pure cultures of fungi. Examine for fungal colonies exhibiting typical microscopic and colonial morphology. Biochemical tests may be required for final identification. The total combined yeasts/moulds count (TYMC) is considered to be equal to the number of CFU found per each plate.
Method logic
Pancreatic digest of casein and peptic digest of animal tissue provide amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Dextrose is an energy source. Agar is the solidifying agent. The high concentration of dextrose and the acidic pH of the medium permit selectivity of fungi. The medium can be supplemented with chloramphenicol to increase bacterial inhibition and recovery of dermatophytes.
Preparation and execution notes
- Preparation: Dehydrated medium Suspend 65 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil for 1 minute shaking frequently until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Medium in bottles Melt the content of the bottle in a water bath at 100°C (losing the cap partially removed) until completely dissolved. Then screw the cap and check the homogeneity of the dissolved medium, if it is the case turning the bottle upside down. Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into Petri dishes.
- Materials required: Standard microbiological supplies and equipment such as: Autoclave, water bath, sterile Petri plates, test tubes, inoculating loops, swabs, incubator,
- Procedure: Ensure there is no visible moisture on the plates before use. For use in medical microbiology Streak the specimen as soon as possible after it is received in the laboratory to obtain isolated colonies. Prepared tubed slants primarily are intended for use with pure cultures for maintenance or other purposes. Incubation conditions may vary according to the type of specimen and the microorganisms being tested for. For use in food, animal feed and water testing. Refer to EN ISO 11133 for specific instructions. For use in industrial microbiology Control of non-sterile products Refer to the
Formula, QC, and performance
- Formula / composition: * (g/litre) Pancreatic Digest of Casein 5.0 Peptic Digest of Animal Tissue 5.0 Dextrose 40.0 Agar 15.0 Final pH 5.6 ± 0.2 at 25°C *Adjusted and/or supplemented as required to meet
- Quality control: organisms. SPECIMENS Clinical specimens should be sampled at the acute stage, before antimicrobial therapy (where possible) and examined as soon as possible after collection. SDA is not suitable for direct inoculation of blood samples. Good laboratory practices for collection, transport and
Risk controls for routine use
- Limitations: Invalid results can be caused by poor specimen quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the principles of the procedures, including its
- Warnings / precautions: 1) For in vitro diagnostic use (IVD). 2) For laboratory professional use only. 3) Operators must be trained and have certain experience. Please read the instructions carefully before using the product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. 4) Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices. 5) Do not use if the product or packaging appears to be damaged. 6) Follow standard
- Storage / shelf life: of the clinical specimens should be applied. Refer to specific guidelines for more information about specimen collection and
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards Results of PBT and vPvB assessment • PBT: Not applicable. • vPvB: Not applicable 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610103
- Catalog/document code matched: 610103, 2024, 11133
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/sabouraud-dextrose-agar-dehydrated-for-microbiology-lab-testing