Product overview
Liofilchem Slanetz Bartley Agar Base Culture Media 610134 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is selective medium for the isolation and enumeration of enterococci in water and other samples. This medium is not intended for use in the diagnosis of disease or other conditions in humans.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Where this product fits
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Result signals and confirmation
Typical colonies show a red, maroon or pink color, either in the centre or throughout the colony. Note: TTC reduction to formazan is not exclusive to enterococci, which may be confirmed by demonstrating aesculin hydrolysis reaction. Following ISO 7899-2, confirm by transferring the membrane and the colonies onto a plate of Bile Aesculin Azide Agar pre-warmed to 44°C. Incubate at 44 ± 0.5°C for 2 hours. Count all typical colonies showing a brown to black color in the surrounding medium as intestinal enterococci.
Method principle
Tryptose provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Yeast extract is a source of vitamins, particularly of group B. Glucose is the fermentable carbohydrate providing carbon and energy. Dipotassium hydrogen phosphate is a buffer. Sodium azide inhibits Gram-negative bacteria and staphylococci. TTC is a redox indicator of bacterial growth, which is colorless in the oxidized form and is reduced to the insoluble red triphenyl formazan. Agar is the solidifying agent.
Bench workflow
- Preparation: Dehydrated medium with TTC Suspend 44.5 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil until completely dissolved. DO NOT AUTOCLAVE. Dispense an appropriate volume into plates, e.g. pour 20 ml of medium into Petri dishes of 90 mm. Dehydrated medium without TTC (agar base) Suspend 44.4 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil until completely dissolved. Autoclave at 121°C for 15 minutes. Cool to 45-50°C. Aseptically add 10 ml of TTC 1% supplement before distributing into Petri dishes.
- Materials required: Standard microbiological supplies and equipment such as: Test tubes, inoculating loops, incubator,
- Procedure: Ensure there is no visible moisture on the plates before use. © Liofilchem - Slanetz Bartley Agar - Rev.3 / 14.07.2025 Page 2 of 4 For the examination of water, filter 100 ml of the sample trough a filter membrane (0.45 μm pore diameter), and transfer this onto the surface of the medium. For other samples, dilute as necessary and spread 0.5 ml over the agar surface. Incubate aerobically at 36 ± 2°C for 40-48 hours. For more details, consult appropriate guidance.
Controls and performance checks
- Formula / composition: * (Per Litre of Purified Water) Tryptose 20.0 g Glucose 2.0 g Yeast Extract 5.0 g Dipotassium Hydrogen Phosphate (K2HPO4) 4.0 g Sodium Azide (NaN3) 0.4 g 2,3,5-triphenyltetrazolium chloride (TTC) 0.1 g Agar 13.0 g Final pH 7.2 ± 0.1 at 25°C *Adjusted and/or supplemented as required to meet
- Performance: specifications. Note: Slanetz Bartley Agar is available both as complete medium and agar base to be used with TTC 1% supplement (see the ORDER INFORMATON section).
Storage, limits, and safety
- Limitations: Invalid results can be caused by poor specimen quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the © Liofilchem - Slanetz Bartley Agar - Rev.3 / 14.07.2025 Page 3 of 4 principles of the procedures, including its
- Warnings / precautions: For professional use only. Operators must be trained and have certain experience. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store prepared plates at 10-25°C away from direct light in their original pack until just prior to use. Avoid quick temperature shifts of plated medium to prevent condensation. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Acute Tox. 4, Acute toxicity, Oral (Category 4); H302 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H302 Harmful if swallowed. Precautionary statement(s) P273 Avoid release to the environment.
- Exposure controls: 8.1 Control parameters • IOELV o Sodium azide; CAS No. : 26628-22-8 ▪ Short-term value: 0.3 mg/m³ ▪ Long-term value: 0.1 mg/m³ ▪ Skin 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. SAFETY DATA SHEET (According to Regulation EC n° 1907/2006 and to Commission Regulation EU 2020/878) Revision: 4 / Revision Date: 17.04.2023 ©Liofilchem s.r.l.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be sp ecially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610134
- Catalog/document code matched: 610134, 2025, 7899
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/slanetz-bartley-agar-base-for-microbiology-lab-testing