Product overview
Liofilchem MR-VP Broth ISO 6785/6579 Culture Media 620032 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is mR-VP BROTH is used for differentiating coliform organisms based on the Methyl Red and Voges-Proskauer tests, as recommended by ISO 6785: 2001 and IDF 93: 2001. The methyl red test is based on the use of a pH indicator to detect acidity when an organism ferments glucose. This is the reason of a development of a red color. The Voges-Proskauer test differentiates microorganisms which ferment carbohydrates to acids from those that ferment them by decarboxylation to acetylmethylcarbinol, which is oxidised to diacetyl by atmospheric oxygen in an environment made basic by KOH. Diacetyl, under the catalytic action of α-naphtol and creatine, is converted into a red complex.
This product is relevant to food safety and environmental monitoring programs where Salmonella screening is part of raw material, finished product, or hygiene verification testing.
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Where this product fits
This product fits screening programs where a negative or presumptive result can affect raw-material release, finished-product hold decisions, or hygiene investigations. The lab should connect the medium to its enrichment sequence, confirmation method, and written rule for when suspect colonies move forward.
Result signals and confirmation
free-flowing, homogeneous. Color: very light beige to light beige. Prepared medium
Bench workflow
- Procedure: Inoculate MR-VP Broth with growth from a single colony. Incubate at 36 ± 1°C for 48 hours. Transfer 3 ml of MR-VP Broth culture to a clean tube. VP Test Prepare the following solutions: - creatine monohydrate: 0.5 g in 100 ml of distilled water; - 5% α-naphtol: 5 g in 100 ml of ethyl alcohol 96%. - 40% KOH: 40 g in 100 ml of distilled water. To 3 ml of broth culture add 2 drops of creatine solution, 3 drops of α-naphtol solution and 2 drops of KOH solution. Gently shake the tube to expose the medium to oxygen. A positive test is indicated by the development of a pink to bright red coloration 15 minutes after the addition of reagents. Methyl Red Test After 5 days of incubation , transfer 5 ml of broth into a clean test tube. Suspend 0.1 g of methyl red in 300 ml of 95% ethyl alcohol, and make up to 500 ml with distilled water.
Controls and performance checks
- Formula / composition: (g/l) Peptospecial 7.0 Dipotassium Phosphate 5.0 Glucose 5.0 Final pH = 7.0 ± 0.2 at 25 °C. DIRECTIONS Suspend 17.0 g of powder in 1 liter of distilled or deionized water. Heat to boiling until completely dissolved. Dispense into final tubes. Sterilize in autoclave at 121°C for 15 minutes.
Storage, limits, and safety
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared tubes at 2-8 °C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74620032
- Catalog/document code matched: 6785, 6579, 620032
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/mr-vp-broth-iso-6785-6579-for-microbiology-lab-testing