Application snapshot
Liofilchem Tryptic Soy Broth VEG Culture Media 610701 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is tryptic Soy Broth (TSB) VEG is a nutritious medium used for the detection, isolation and cultivation of fastidious and nonfastidious microorganisms including bacteria and fungi from various types of sample. This medium contains vegetable peptones and is free of any ingredient of animal origin. That eliminates regulatory issues and concerns related to use of animal-derived products such as contamination risk with TSE/BSE. TSB VEG is an alternative to Casein Soya Bean Digest Broth recommended in the harmonised USP/EP/JP method for sterility testing and microbiological examination of non-sterile products as well as by the CLSI for inoculum
This product is relevant to surface hygiene and environmental monitoring. It is best positioned for food plants, cleanrooms, cosmetics facilities, and sanitation teams that need a controlled, documentable microbiology workflow rather than a generic consumable.
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Application fit for the lab
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Expected colonies or reaction pattern
The presence of turbidity compared to an uninoculated control or a pellicle formation indicate microbial growth. Subculture to suitable solid media for complete identification of the isolated colonies. If the material being tested renders the medium turbid and a visual examination is not possible at the end of the incubation period, subculture to fresh TSB VEG or onto appropriate solid media to ensure that turbidity is caused by the sample only and it is not a result of microorganisms multiplying in the broth.
Principle behind the medium
Wheat peptone (a certified non-animal peptone) and papaic digest of soya bean provide amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Sodium chloride maintains osmotic balance in the medium. Dipotassium phosphate is a buffering agent. Glucose is an energy source.
How to build it into the SOP
- Preparation: in antibiotic susceptibility testing. The microbiological
- Procedure: For use in industrial microbiology, inoculate the sample or material to be tested into the medium. Incubate under appropriate atmosphere at 30-35°C for 18-72 hour (bacteria) and at 20-25°C for a up to 5 days (fungi). For sterility testing, the way of inoculation depends on the type and size of test material. If membrane filtration is carried out, a suitable diluent should be used. For direct inoculation, it is recommended a minimum 1:10 dilution of the sample to the culture medium. Incubate at 20-25°C for 14 days. For media fill test in biopharmaceutical manufacturing, two incubation temperatures are used. The initial incubation at 20-25°C for 7 days, and then at 30-35°C for further 7 days.
Composition and QC checkpoints
- Formula / composition: * (g/l) *Adjusted and/or supplemented as required to meet
- Performance: and applications of TSB VEG are the same as the standard TSB.
Handling limits and documentation
- Warnings / precautions: For professional use only. Operators must be trained and have certain experience in the laboratory methods. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration. Instructions For Use Tryptic Soy Broth VEG Liquid medium for the isolation and cultivation of a wide variety of organisms.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classif ication in any hazard class in accordance to Regula tion (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non-recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74610701
- Catalog/document code matched: 610701, 2022
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/tryptic-soy-broth-veg-for-microbiology-lab-testing