Product overview
Liofilchem EE Broth-Mossel (Harm.EP) Culture Media 620017 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is enterobacteriaceae Enrichment Broth-Mossel is a selective medium used for the detection of bile-tolerant Gram-negative bacteria in food and other materials of sanitary importance. This medium complies with the recommendations of the harmonized method in the United States Pharmacopoeia (USP), European Pharmacopoeia (EP) and Japanese Pharmacopoeia (JP) for the microbiological examination of nonsterile products.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Where this product fits
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Result signals and confirmation
Turbidity of EE Broth-Mossel indicates microbial growth; acid production causes a color change of the medium to yellow. No growth of colonies on Violet Red Bile Glucose Agar is reported as absence of bile-tolerant Gram-negative bacteria. Growth of colonies constitutes a positive result and the probable number of bacteria is determined from the table below. MPN Table.
Method principle
Pancreatic digest of gelatin provides amino acids, nitrogen, carbon, vitamins and minerals for organisms growth. Glucose is the fermentable carbohydrate. Ox bile and brilliant green are selective agents effective against Gram-positive cocci. Potassium phosphate and sodium phosphate act as buffer.
Bench workflow
- Preparation: Dehydrated medium Suspend 43.4 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. DO NOT AUTOCLAVE.
- Procedure: As in the Pharmacopoeia, prepare the sample using a 1 in 10 dilution of not less than 1 g of the product to be examined by choosing Tryptic Soy Broth (ref. 24513 or 452080) as diluent and incubate at 20-25°C for 2-5 hour to resuscitate bacteria. For qualitative test (test for absence), transfer the volume of the pre-enrichment broth corresponding to 1 g of the product to be examined to EE Broth-Mossel. For quantitative test, enumerate Enterobacteriaceae found per milliliter or per gram of test sample by using the Most Probable Number (MPN)
Controls and performance checks
- Formula / composition: (g/l) Pancreatic Digest of Gelatin 10.0 Glucose Monohydrate 5.0 Dehydrated Ox Bile 20.0 Potassium Dihydrogen Phosphate 2.0 Disodium Hydrogen Phosphate, Anhydrous 6.4* Brilliant Green 0.015 Final pH 7.2 ± 0.2 at 25°C * Equivalent to 8.0 g of Disodium Hydrogen Phosphate Dihydrate.
- Quality control: The medium is inoculated with the microbial strains indicated in the QC table. Inoculum for productivity: ≤100 CFU. Inoculum for selectivity: >100 CFU. Incubation conditions: 18-24 h at 30-35°C (Pharmacopoeia growth promotion). QC Table. Microorganism Specification Escherichia coli ATCC 8739 Good growth Pseudomonas aeruginosaATCC 9027 Good growth Staphylococcus aureus ATCC 6538 Inhibition WARNING AND
Storage, limits, and safety
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for professional use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store bottles and prepared plates at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the sub stance or mixture This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to o fficial regulations administrative. Offer surplus a nd non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74620017
- Catalog/document code matched: 620017, 2016
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/ee-broth-mossel-harm-ep-for-microbiology-lab-testing