Laboratory use profile
Liofilchem Tryptic Soy Agar + Lecithin Culture Media 610403 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is non-selective solid medium used for the monitoring of microbial contamination. This medium is not intended for use in the diagnosis of disease or other conditions in humans.
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Use case and sample types
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Decision criteria after incubation
Observe for the formation of colonies and record the number of CFU per plate. The user is responsible for
Method logic
Casein peptone and soy peptone provide amino acids, nitrogen, carbon, minerals, vitamins and other nutrients which support the growth of microorganisms. Sodium chloride maintains the osmotic balance of the medium. Lecithin is a neutralizer particularly effective against quaternary ammonium compounds. Agar is the solidifying agent.
Preparation and execution notes
- Preparation: Suspend 40.7 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50°C and pour in Petri dishes or contact plates.
- Materials required: Standard microbiological supplies and equipment such as: Autoclave, water bath, sterile Petri plates and/or Contact plates, air sampler, test tubes, inoculating loops, swabs, incubator,
- Procedure: For active air monitoring, place the contact plate, lid up, in the air sampler, remove the lid of the plate and sample a specific volume of air according to laboratory
Formula, QC, and performance
- Formula / composition: * (Per Litre of Purified Water) Casein Peptone 15.0 g Soy Peptone 5.0 g Sodium Chloride 5.0 g Lecithin 0.7 g Agar 15.0 g Final pH 7.3 ± 0.2 at 25°C *Adjusted and/or supplemented as required to meet
Risk controls for routine use
- Limitations: Invalid results can be caused by poor sample quality, improper sample collection, improper transportation, improper laboratory processing, or a limitation of the testing technology. The operator should understand the principles of the procedures, including its
- Warnings / precautions: © Liofilchem - Tryptic Soy Agar + Lecithin - Rev.0 / 13.03.2024 Page 3 of 4 For professional use only. Operators must be trained and have certain experience. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters no data available 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices. Respiratory protection: It is advisable wear dust mask.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74610403
- Catalog/document code matched: 610403, 2024
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/tryptic-soy-agar-lecithin-for-microbiology-lab-testing