Laboratory use profile
Liofilchem Azide Dextrose Broth Culture Media 620003 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is aZIDE DEXTROSE BROTH is a selective broth used for presumptive identification and enumeration of fecal streptococci in waters and foodstuffs. Their presence is indicated by turbidity of the broth and evaluated according to the most probable number method (MPN).
This product is relevant to water and beverage laboratories where filtration, enrichment, colony recovery, and indicator-organism interpretation are tied to release or investigation decisions.
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Use case and sample types
This product fits environmental monitoring where repeatable sampling and neutralization are more important than a single isolated result. The lab should connect the medium to surface type, disinfectant residues, incubation plan, alert/action limits, and trend review.
Decision criteria after incubation
free-flowing, homogeneous. Color: beige. Prepared medium
Preparation and execution notes
- Procedure: Inoculate a series of Azide Dextrose Broth tubes with approximately graduated quantities of sample. Use quantities of 10 ml or less. Use double strength broth for 10 ml inocula. Consult an appropriate reference for suggested sample sizes. Incubate inoculated tubes at 36 ± 1° C for 20-48 hours. Examine each tube for turbidity at the end of 24 ± 2 hours. If no turbidity is evident, reincubate and read again at the end of 48 ± 3 hours.
Formula, QC, and performance
- Formula / composition: (g/l) Tryptose 15.0 Beef Extract 4.5 Glucose 7.5 Sodium Chloride 7.5 Sodium Azide 0.2 Final pH = 7.2 ± 0.2 at 25 °C. DIRECTIONS Suspend 34.7 g (single strength medium) or 69.4 g (double strength medium) of powder in 1 liter of distilled or deionized water. Heat until completely dissolved. Dispense into final containers. Sterilize in autoclave at 121 °C for 15 minutes.
Risk controls for routine use
- Limitations: Azide Dextrose Broth is used for presumptive detection of fecal contamination and further culture tests must be done for confirmation.
- Warnings / precautions: Azide Dextrose Broth contains sodium azide. This substance is harmful by inhalation and if swallowed, irritating to eyes, respiratory system and skin. Consult safety data sheet for further details. Sodium azide reacts with many metals, especially copper, to produce explosive metal azides. Dehydrated culture medium Rev. 0 - 3rd Ed.
- Storage / shelf life: The powder is very hygroscopic: store the powder at 10-30 °C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared tubes at 2-8 °C.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Acute Tox. 4, Acute toxicity, Oral (Category 4); H302 Aquatic Chronic 3, Chronic aquatic toxicity (Category 3); H412 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Warning Hazard statement(s) H302 H412 Harmful if swallowed. Harmful to aquatic life with long lasting effects.
- Exposure controls: 8.1 Control parameters • Sodium azide (CAS 26628-22-8) Indicative occupational exposure limit values (IOELV) Short term: 0,3 mg/m3 Long term: 0,1 mg/m3 SAFETY DATA SHEET (According to Regulation EC n° 1907/2006 and to Commission Regulation EU 2020/878) Revision: 4 / Revision Date: 20.11.2023 ©Liofilchem s.r.l. Via Scozia 64026, Roseto degli Abruzzi (TE) Italy - Tel +39 0858930745 - Fax +39 0858930330 MSDS Ref.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product m ust be sp ecially treated adhering to official regulations administrative. Offer surplus and non - recyclable solutions to a licensed
What to verify before ordering
- Kormay SKU: KB74620003
- Catalog/document code matched: 620003
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/azide-dextrose-broth-for-microbiology-lab-testing