Product overview
Liofilchem Listeria Fraser Broth Culture Media 620166 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is demi Fraser Broth is a liquid medium used with supplements for the secondary enrichment of L monocytogenes and Listeria spp from food, animal feeding and environmental samples in the area of food production and food handling. The medium is completed after the addition of Listeria Fraser Supplement which is available in the following forms: •Freeze-dried (ref. 81028)•Liquid (ref. 80304)Both items contain Ammonium Iron(III) Citrate.
This product supports Listeria workflows in ready-to-eat food, dairy, meat, and environmental monitoring programs where early presumptive recovery must lead into confirmation.
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Where this product fits
Use this product where the lab must protect a recovery step before confirmation. For ready-to-eat food and environmental samples, the important decision is not only whether growth appears, but whether the enrichment or plating step gives the organism enough opportunity to recover from stress, competing flora, preservatives, or cold-chain exposure.
Result signals and confirmation
A blackening of Demi Fraser Broth can be observed after incubation. Blue-green colonies with or without halo on O.A. Listeria Agar are considered presumptive Listeria spp. Typical colonies of L. monocytogenes are surrounded by an opaque halo. For confirmation, subculture onto appropriate non-selective agar, e.g. Blood Agar, Nutrient Agar, TSYEA (ref. 10432). Then, carry out confirmation tests including a positive and negative control.
Method principle
Enzymatic digest of animal tissues, enzymatic digest of casein and meat extract provide nitrogen, vitamins, minerals and amino acids for organisms growth. Yeast extract is a source of vitamins, particularly of B-group. Sodium chloride maintains the osmotic balance of the medium and in a so high concentration inhibits enterococci. Potassium and sodium phosphates act as buffer system. Aesculin is hydrolyzed by all Listeria species to aesculetin. Lithium chloride is inhibitory for the accompanying flora. Acriflavine and nalidixic acid areselective agents.Ferric ions provided by ammonium iron(III) citrate will react with aesculetin producing a blackening of the medium.
Bench workflow
- Preparation: Suspend 55 g of powder in 1 liter of deionized or distilled water. Bring to boil and shake until completely dissolved. Sterilize at 121°C for 15 minutes. Cool up to 45-50°C. Aseptically, add the content of 2 vials of Listeria Fraser Supplement. NOTE: Either ref. 81028 or ref. 80304 can be chosen. The latter is a ready to use liquid solution whereas ref. 81028 needs to be reconstituted with water before use.
- Procedure: 1.Add sample to Demi Fraser Broth (ref. 452401) to prepare a 10-fold dilution (w/v or v/v). Incubate at 30 ± 1°C for 25 ± 1 h. 2.Transfer 0.1 ml of the primary enrichment culture into 10 ml of Listeria Fraser Broth. Incubate at 37 ± 1°C for 24 ± 2 h. 3.Streak from both primary and secondary enrichments onto O.A. Listeria Agar (ref. 10620) to obtain well-separated colonies. Incubate at 37 ± 1°C for 24 ± 2 h and for an additional 24 ± 2 h. 4.Use the selective enrichments to inoculate a second selective medium, e.g. Listeria Palcam Agar (ref. 10041), Listeria Oxford Agar(ref. 61016). Refer to the relevant technical sheet for further details.
Controls and performance checks
- Formula / composition: (g/l)Enzymatic Digest of Animal Tissues 5.0Enzymatic Digest of Casein 5.0Meat Extract 5.0Yeast Extract 5.0Sodium Chloride 20.0Disodium Phosphate, Anhydrous 9.6*Potassium Dihydrogen Phosphate 1.35Aesculin 1.0Lithium Chloride 3.0Nalidixic Acid 0.02Acriflavine 0.025Final pH 7.2 ± 0.2 at 25°C*Equivalent to 12.0 g of Disodium Hydrogen Phosphate, Dihydrate.
- Quality control: 1.Control of general characteristics, label and print2.Microbiological controlIncubation Conditions: 24 ± 2 h / 37 ± 1°CInoculum for productivity: ≤100 CFUMicroorganismSpecificationListeria monocytogenes serovar 4b + Escherichia coli + Enterococcus faecalisWDCM 00021WDCM 00013WDCM 00009Blackening of the medium,>10 colonies on O.A.
- Performance: testing of culture media.4.Rapporto ISTISAN 96/35. ISSN 1123-3117. Metodi di analisi per il controllo microbiologico degli alimenti. 5.Normalisation Francaise, AFNOR (1993) V08-55. 6.Fraser. J.A and Sperber W.H (1988) J. Food Prot , 51, 762-765. LIOFILCHEM S.r.l.Via Scozia, Zona Ind.le - 64026, Roseto degli Abruzzi (TE) - ITALYTel +39 0858930745 Fax +39 0858930330 www.liofilchem.com TECHNICAL SHEETTS 610166Rev.1 / 23.11.2018Page 3 of 3PRODUCT SPECIFICATIONSNAMEListeria Fraser Broth PRESENTATIONDehydrated medium
Storage, limits, and safety
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is notclassified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is designed for professional use only and must be used by properly trained operators.
- Storage / shelf life: CONDITIONS The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2-8°C away from light.WARNING AND
- Waste disposal: OF WASTEDisposal of waste must be carried out according to the national and local regulations in force.LIOFILCHEM S.r.l.Via Scozia, Zona Ind.le - 64026, Roseto degli Abruzzi (TE) - ITALYTel +39 0858930745 Fax +39 0858930330 www.liofilchem.com TECHNICAL SHEETTS 610166Rev.1 / 23.11.2018Page 2 of 3REFERENCES1.ISO 11290-1:2017.Microbiology of the food chain ‒ Horizontal method for the detection and enumeration of Listeria monocytogenes and Listeria spp.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] This product does not meet the criteria for classification in any hazard class in accordance to Regulation (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws.
- Exposure controls: 8.1 Control parameters • Lithium Chloride powder (CAS 7447-41-8) Long-term worker DNEL – dermal (systemic effects - acute) – 100 mg/m3 Long-term worker DNEL – dermal (systemic effects - chronic) – 73,2 mg/m3 Long-term worker DNEL – inhalation (systemic effects - acute)– 30 mg/m3 Long-term worker DNEL – inhalation (systemic effects - chronic)– 10 mg/m3 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be sp ecially treated adhering to official regulations administrative. Offer surp lus and non -recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74620166
- Catalog/document code matched: 620166, 11290, 81028, 80304
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/listeria-fraser-broth-for-microbiology-lab-testing