Product overview
Liofilchem WL Differential Agar Culture Media 610400 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is wL (Wallerstein Laboratory) Differential Agar is a medium used for the selective isolation and enumeration of spoilage bacteria in brewing and fermentation processes. This medium consists of WL Nutrient Agar, modified by adding cycloheximide (actidione) to prevent the growth of brewing yeast.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Where this product fits
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Result signals and confirmation
of Dehydrated Medium: Free-flowing, homogeneous, light beige with greenish tin
Method principle
Tryptone provides amino acids, nitrogen, carbon, vitamins and minerals. Yeast extract is a source of vitamins, particularly of B-group. Dextrose is the fermentable carbohydrate. Monopotassium phosphate acts as a buffer. Potassium chloride, calcium chloride, and ferric chloride are essential ions and help to maintain osmotic balance of the medium. Magnesium sulfate and manganese sulfate are sources of divalent cations. Bromcresol green is a pH indicator. Cycloheximide is the selective agent which is included into the medium to suppress yeasts and moulds while permitting the growth of bacteria. Agar is the solidifying agent.
Bench workflow
- Procedure: AND EVALUATION Inoculate the medium using the pour plate
Controls and performance checks
- Formula / composition: * (g/litre) *Adjusted and/or supplemented as required to meet
Storage, limits, and safety
- Warnings / precautions: For professional use only. Operators must be trained and have certain experience in the laboratory methods. Please read the instructions carefully before using this product. Reliability of assay results cannot be guaranteed if there are any deviations from the instructions in this document. Consult the Safety Data Sheet (SDS) for information regarding hazards and safe handling practices.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store prepared plates at 2-8°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration. WL Differential Agar Selective medium for the cultivation and enumeration of bacteria in the brewing and other fermentation industries.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture This product does not meet the criteria for classif ication in any hazard class in accordance to Regula tion (EC) No. 1272/2008. concerning the classification, labelling and packaging of substances and mixtures. 2.2 Label elements The product does not need to be labeled in accordance with EC directives or respective national laws. 2.3 Other hazards - none 3.
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non-recyclable solutions to a licensed
Ordering and documentation checks
- Kormay SKU: KB74610400
- Catalog/document code matched: 610400, 2022, 0025
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/wl-differential-agar-for-microbiology-lab-testing