Application snapshot
Liofilchem Yeast Glucose Chloramphenicol Agar Culture Media 610070 is a Liofilchem microbiology product supplied by Kormay Biomedicals. Its practical role is yeast Glucose Chloramphenicol Agar (YGC) is a medium used for the selective isolation and enumeration of fungi. This medium complies with the recommendations of the International Dairy Federation (FIL-IDF) for the examination of milk and milk products.
This product belongs in fungal or yeast workflows where recovery, differentiation, or susceptibility testing depends on controlled media, incubation, and disciplined result reading.
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Application fit for the lab
This product is most useful when spoilage organisms or yeasts need controlled recovery and readable morphology. The lab should define incubation conditions, what counts as typical growth, and how suspect isolates are confirmed before the result is used for shelf-life or investigation decisions.
Expected colonies or reaction pattern
Count colonies on all plates containing 15-300 colonies. Distinguish yeasts from molds by colony morphology. Report the count as yeasts and moulds per gram or per milliliter of sample allowing for dilution factors.
Principle behind the medium
Yeast extract provide basic nutrients and is a good source of vitamins, particularly of B-group. Glucose is the fermentable carbohydrate. Chloramphenicol is the selective agent which inhibit the accompanying bacterial flora resulting in better recovery of injured fungal cells. Agar is the solidifying agent.
How to build it into the SOP
- Preparation: Dehydrated medium Suspend 43.1 g of the powder in 1 liter of distilled or deionized water. Mix well. Heat to boil shaking frequently until completely dissolved. Sterilize in autoclave at 121°C for 15 minutes. Medium in bottles Melt the content of the bottle in a water bath at 100°C (loosing the cap partially removed) until completely dissolved. Then screw the cap and check the homogeneity of the dissolved medium, if it is the case turning the bottle upside down. Cool at 45-50°C, mix well avoiding foam formation and aseptically distribute into Petri dishes.
- Procedure: Prepare sample dilutions in 1/4-strength Ringer’s Solution (ref. 81059) or other suitable diluent. Inoculate the medium by pour plating or spread plating method. Large volumes of sample may be concentrated by membrane filtration. Filters are then placed on YGC agar plates of 60 mm diameter. Incubate plates aerobically at 25 ± 2°C for up to 5 days.
Composition and QC checkpoints
- Formula / composition: (g/l) Yeast Extract 5.0 Glucose 20.0 Chloramphenicol 0.1 Agar 18.0 Final pH 6.6 ± 0.2 at 25°C
- Quality control: Plates are inoculated with the microbial strains indicated in the QC table. Inoculum for productivity: 50-100 CFU. Inoculum for selectivity: 104-106 CFU. Incubation conditions: aerobically at 25 ± 2°C for up to 5 days. QC Table. Microorganism Growth Aspergillus brasiliensis ATCC 16404 Good Candida albicans ATCC 10231 Good Saccharomyces cerevisiaeATCC 9763 Good Escherichia coli ATCC 25922 Inhibited WARNING AND
Handling limits and documentation
- Warnings / precautions: The product does not contain hazardous substances in concentrations exceeding the limits set by current legislation and therefore is not classified as dangerous. It is nevertheless recommended to consult the safety data sheet for its correct use. The product is intended for professional use only and must be used by properly trained operators.
- Storage / shelf life: The powder is very hygroscopic, store the powder at 10-30°C, in a dry environment, in its original container tightly closed. Store bottles and prepared plates at 10-25°C away from light. Do not use the product beyond its expiry date on the label or if product shows any evidence of contamination or any sign of deterioration.
- Waste disposal: of waste must be carried out according to national and local regulations in force.
Safety and documentation points
- Hazards: 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Carc. 1A, carcinogenicity(Category 1A); H350 2.2 Label elements Labelling according Regulation (EC) No 1272/2008 [CLP] Pictogram Signal word Danger Hazard statement(s) H350 May cause cancer Precautionary statement(s) P202 P280 P308+P313 Do not handle until all safety
- Exposure controls: 8.1 Control parameters Components with workplace control parameters Contains no substances with occupational exposure limit value. 8.2 Exposure controls Exposure controls Handle in accordance with good hygiene and safety practice. Wash hands before breaks and at the end of workday. Personal protective equipment Eyes/Face Protection: It is advisable wear safety goggles Skin protection: Handle with gloves Body Protection: Use protective clothes in accordance with laboratory good practices.
- Disposal: 13.1 Waste treatment methods Product Not be disposed of with household waste. Do not discharge into sewers. The product must be specially treated adhering to official regulations administrative. Offer surplus and non -recyclable solutions to a licensed
Before adding it to the cart
- Kormay SKU: KB74610070
- Catalog/document code matched: 610070, 2020
Before ordering, confirm the current IFU, SDS/MSDS, batch QC certificate or COA requirements, storage temperature, shelf life after receipt, and whether your SOP needs method verification before routine use.
Order / request documentation: https://www.kormay.ca/products/yeast-glucose-chloramphenicol-agar-unit-500-g